Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Histone Modification02:32

Histone Modification

16.1K
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
16.1K
Histone Modification02:32

Histone Modification

4.5K
4.5K
Spreading of Chromatin Modifications02:25

Spreading of Chromatin Modifications

9.4K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
9.4K
Histone Variants at the Centromere02:30

Histone Variants at the Centromere

5.0K
Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3...
5.0K
Alterations in Respiration II01:30

Alterations in Respiration II

1.8K
There are numerous types of normal and abnormal respiration. Based on ventilatory movements, breathing patterns are classified as regular, deep, or shallow. Examples include Biot's breathing, Cheyne-Stokes respiration, Kussmaul's breathing, hyperventilation, and hypoventilation. Each pattern is clinically significant and aids in evaluating patients.
In Biot's breathing, the respiratory rate and depth are irregular, alternating between periods of deep gasping and apnea. Common causes...
1.8K
Altered States of Awareness01:06

Altered States of Awareness

1.1K
Altered states of consciousness represent significant deviations from one's normal mental state. These deviations can range from subtle changes in awareness to profound transformations in perception, thought processes, and sensory experiences. Altered states of consciousness can be triggered by various factors, including drug use, meditation, hypnosis, illness, or even intense fatigue.
The ingestion of substances like stimulants or hallucinogens leads to chemical alterations in the brain...
1.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Description of nine novel psychrophilic <i>Pseudarthrobacter</i> species from glaciers.

International journal of systematic and evolutionary microbiology·2026
Same author

Editorial: Molecular mechanisms and therapeutic strategies of head and neck disease.

Frontiers in cell and developmental biology·2026
Same author

Neuro-Immune Crosstalk: Molecular Mechanisms, Biological Functions, Diseases, and Therapeutic Targets.

MedComm·2026
Same author

Disruption of goat airway epithelial barrier function by caprine parainfluenza virus type 3 infection in an ALI model.

Veterinary microbiology·2026
Same author

Description of 15 novel species within genus <i>Janthinobacterium</i> isolated from glaciers.

International journal of systematic and evolutionary microbiology·2026
Same author

Ten novel psychrophilic <i>Flavobacterium</i> species from Tibetan Plateau glaciers define a cryospheric lineage with global cold-origin relatives.

International journal of systematic and evolutionary microbiology·2026

Related Experiment Video

Updated: Jan 27, 2026

Characterizing Histone Post-translational Modification Alterations in Yeast Neurodegenerative Proteinopathy Models
08:33

Characterizing Histone Post-translational Modification Alterations in Yeast Neurodegenerative Proteinopathy Models

Published on: March 24, 2019

7.9K

Multiple superovulations alter histone modifications in mouse early embryos.

Shou-Bin Tang1, Lei-Lei Yang1, Ting-Ting Zhang2

  • 1College of Animal Science and Technology, Institute of Reproductive Sciences, Qingdao Agricultural University, Qingdao, People's Republic of China.

Reproduction (Cambridge, England)
|March 19, 2019
PubMed
Summary

Repeated superovulation negatively impacts mouse early embryo development and fertilization rates. Multiple superovulations alter key histone modifications, affecting epigenetic reprogramming crucial for embryonic development.

More Related Videos

Detection of Histone Modifications in Plant Leaves
07:08

Detection of Histone Modifications in Plant Leaves

Published on: September 23, 2011

25.4K
Author Spotlight: Enhancements in Gene Expression Regulation Research
07:10

Author Spotlight: Enhancements in Gene Expression Regulation Research

Published on: September 15, 2023

2.6K

Related Experiment Videos

Last Updated: Jan 27, 2026

Characterizing Histone Post-translational Modification Alterations in Yeast Neurodegenerative Proteinopathy Models
08:33

Characterizing Histone Post-translational Modification Alterations in Yeast Neurodegenerative Proteinopathy Models

Published on: March 24, 2019

7.9K
Detection of Histone Modifications in Plant Leaves
07:08

Detection of Histone Modifications in Plant Leaves

Published on: September 23, 2011

25.4K
Author Spotlight: Enhancements in Gene Expression Regulation Research
07:10

Author Spotlight: Enhancements in Gene Expression Regulation Research

Published on: September 15, 2023

2.6K

Area of Science:

  • Reproductive Biology
  • Developmental Biology
  • Epigenetics

Background:

  • Repeated superovulation is used in assisted reproduction but its effects on early embryos are not well understood.
  • Epigenetic reprogramming is vital for early embryonic development and sensitive to environmental changes.

Purpose of the Study:

  • To investigate the impact of multiple superovulations on histone modifications in early mouse embryos.
  • To assess the effects on fertilization rates, embryo development, and key developmental gene expression.

Main Methods:

  • CD1 mice underwent 0, 1, 3, or 5 superovulation cycles at 7-day intervals.
  • Fertilization rates, early embryo development, and blastocyst gene expression (Oct4, Sox2, Nanog) were analyzed.
  • Immunofluorescence assessed histone modifications (H4K12ac, H4K16ac, H3K9me2, H3K27me3).
  • mRNA levels of histone-modifying enzymes were quantified.

Main Results:

  • Repeated superovulation significantly decreased fertilization and early embryo development rates.
  • Histone acetylation (H4K12ac, H4K16ac) was reduced, while methylation (H3K9me2, H3K27me3) increased with more superovulations.
  • Expression of key developmental genes (Oct4, Sox2, Nanog) was altered.
  • mRNA levels of HDACs (Hdac1, Hdac2) and G9a increased, while demethylases (Kdm6a, Kdm6b) decreased.

Conclusions:

  • Multiple superovulations adversely affect early mouse embryo development and epigenetic profiles.
  • Altered histone modifications, including reduced acetylation and increased methylation, are key consequences.
  • These epigenetic changes may underlie the observed developmental deficits following repeated superovulation.