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Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

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Optimizing Suitable Antibiotics for Bacterium Control in Micropropagation of Cherry Rootstock Using a Modified Leaf

Chenglin Liang¹, Rendun Wu², Yu Han³

¹College of Horticulture, Northwest A&F University, Yangling 712100, China. ljl2015lcl@163.com.

Plants (Basel, Switzerland)

|March 20, 2019

Summary

This summary is machine-generated.

Tetracycline effectively controls bacterial contaminants in plant tissue culture. A minimal inhibitory concentration (MIC) of 1.0 µg mL⁻¹ was identified, showing minimal negative effects on cherry explants at this level.

Keywords:

E test antibiotics bacterial contaminants disk diffusion in vitro plant culture

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Area of Science:

Plant Biotechnology
Microbiology
Agricultural Science

Background:

Bacterial contamination poses a significant challenge to in vitro plant culture success.
Effective methods are needed to control microbial contaminants without harming plant tissues.

Purpose of the Study:

To identify optimal antibiotics for controlling bacterial contaminants in cherry rootstock 'Gisela 6' cultures.
To determine the minimal inhibitory concentration (MIC) of effective antibiotics.
To assess the impact of antibiotics on plant explant health.

Main Methods:

Disk diffusion assay to screen antibiotic efficacy against isolated bacteria.
E test to quantify the minimal inhibitory concentration (MIC) of the most effective antibiotic.
Measurement of chlorophyll content, Fv/Fm values, and enzyme activity (superoxide dismutase, peroxidase) to evaluate explant response.

Main Results:

Tetracycline demonstrated the highest efficacy in inhibiting bacterial growth.
The MIC for tetracycline was determined to be 1.0 µg mL⁻¹.
Low concentrations of tetracycline (≤ 1.0 µg mL⁻¹) had minimal negative effects on cherry explants, while higher concentrations caused significant damage.

Conclusions:

The disk diffusion and E test methods are valuable for identifying effective antibiotics and their MICs for plant tissue culture.
Tetracycline at 1.0 µg mL⁻¹ is a promising agent for controlling bacterial contamination in 'Gisela 6' cherry rootstock cultures with minimal phytotoxicity.