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Updated: Jan 27, 2026
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Quantitation of Endothelial Cell Adhesiveness In Vitro
Published on: June 18, 2015
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ROCK2 Regulates Monocyte Migration and Cell to Cell Adhesion in Vascular Endothelial Cells
Yusuke Takeda1, Keiichiro Matoba2, Daiji Kawanami3
1Division of Diabetes, Metabolism and Endocrinology, Department of Internal Medicine, Jikei University School of Medicine, 3-25-8 Nishishinbashi, Minato-ku, Tokyo 105-8461, Japan. ms05-takeda@jikei.ac.jp.
International Journal of Molecular Sciences
|March 20, 2019
Summary
Rho-kinase 2 (ROCK2) is a key regulator of endothelial inflammation, controlling monocyte migration and adhesion. Targeting ROCK2 may offer a potential therapeutic strategy for attenuating atherosclerosis.
Area of Science:
- Molecular Biology
- Cell Biology
- Immunology
Background:
- Rho-kinase (ROCK) signaling regulates cellular functions like actin cytoskeleton organization, cell adhesion, and migration.
- Lysophosphatidic acid (LPA), a pro-inflammatory lipid, activates the Rho/ROCK pathway, inducing chemokines and cell adhesion molecules (CAMs).
Purpose of the Study:
- To elucidate the precise mechanism by which ROCK regulates LPA-induced expressions and functions of chemokines and CAMs.
- To investigate the specific roles of ROCK isoforms in endothelial inflammation.
Main Methods:
- ROCK blockade to assess effects on IκBα and NF-κB RelA/p65 phosphorylation and nuclear translocation.
- Small interfering RNA (siRNA)-mediated knockdown of ROCK isoforms in human aortic endothelial cells (HAECs).
- Assessment of monocyte chemoattractant protein-1 (MCP-1) and E-selectin expression.
- Evaluation of LPA-induced monocytic migration and monocyte adhesion to endothelial cells.
Main Results:
- ROCK blockade reduced LPA-induced phosphorylation of IκBα and NF-κB RelA/p65, inhibiting nuclear translocation.
- LPA induced MCP-1 and E-selectin expression via ROCK2 in HAECs.
- ROCK2, not ROCK1, controlled LPA-induced monocytic migration and adhesion to endothelial cells.
Conclusions:
- ROCK2 is a key regulator of endothelial inflammation.
- Targeting endothelial ROCK2 may be effective in attenuating atherosclerosis.
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