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Subcutaneous Infection of Methicillin Resistant Staphylococcus Aureus MRSA
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Subcutaneous Infection of Methicillin Resistant Staphylococcus Aureus MRSA

Published on: February 9, 2011

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Genetic Profiling and Comparison of Human and Animal Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from

Jelena Asanin1, Dusan Misic2, Ksenija Aksentijevic3

  • 1Innovation Center of Faculty of Technology and Metallurgy, University of Belgrade, 11000 Beograd, Serbia. jelenaasanin@gmail.com.

Antibiotics (Basel, Switzerland)
|March 20, 2019
PubMed

Insights

This study characterized methicillin-resistant Staphylococcus aureus (MRSA) from humans and animals in Serbia. Human MRSA clones were found in companion animals, indicating potential for zoonotic transmission of antibiotic-resistant bacteria.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Public Health

Background:

  • Methicillin-resistant Staphylococcus aureus (MRSA) poses a significant threat to public health due to its resistance to antibiotics.
  • Understanding the genetic diversity and transmission dynamics of MRSA between humans and animals is crucial for effective control strategies.
  • Surveillance of MRSA in both human and companion animal populations can reveal potential reservoirs and transmission routes.

Purpose of the Study:

  • To characterize a collection of MRSA isolates from human and companion animal origins in Serbia.
  • To investigate the presence of antibiotic resistance determinants, biocide resistance determinants, and virulence genes in these MRSA isolates.
  • To determine the genetic relatedness and clonal types of MRSA isolates using various typing methods.

Main Methods:

  • Polymerase Chain Reaction (PCR) was used to detect resistance and virulence genes.
  • Typing methods included staphylococcal cassette chromosome mec (SCCmec), spa, dru, multiple locus variable number of tandem repeat analyses (MLVA), and multilocus sequence typing (MLST).
  • eBURST analysis was employed to infer population structures and clonal lineages.

Main Results:

  • Human MRSA isolates predominantly showed resistance to gentamicin, erythromycin, clindamycin, and ciprofloxacin, while all isolates were susceptible to teicoplanin and linezolid.
  • SCCmec type III was common in human isolates, whereas animal isolates had mostly nontypeable SCCmec elements.
  • Human-associated MRSA clones (ST5, ST45, ST239) were identified in companion animals, suggesting anthropozoonotic transmission.

Conclusions:

  • The study identified specific antibiotic resistance profiles and genetic types of MRSA in Serbia.
  • The presence of human-associated MRSA clones in companion animals highlights the potential for zoonotic transmission.
  • Continuous monitoring and understanding of MRSA epidemiology in both human and animal populations are essential for public health interventions.
Keywords:
MLSTMLVAMRSASCCmec typinganimalsantibiotic resistancedru typinghumansspa typing

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