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A toolset to study functions of Cytosolic non-specific dipeptidase 2 (CNDP2) using Drosophila as a model organism

Evgeniya N Andreyeva1, Anna A Ogienko2,3, Tatiana D Dubatolova2

  • 1Institute of Molecular and Cellular Biology, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090, Russia. andreeva@mcb.nsc.ru.

BMC Genetics
|March 20, 2019
PubMed
Abstract

Insights

Researchers developed tools to study the CNDP2 gene in fruit flies. The dCNDP2 gene is not essential for fly survival, but its protein localizes to the nucleus and cytoplasm, suggesting potential roles.

Area of Science:

  • Genetics
  • Molecular Biology
  • Developmental Biology

Background:

  • The CNDP2 gene's expression is altered in human cancers, but its role in cell growth is unclear.
  • The function of CNDP2 orthologs in model organisms, like Drosophila melanogaster, is largely unknown.
  • The specific function of the D. melanogaster CNDP2 ortholog (dCNDP2) remains uncharacterized.

Purpose of the Study:

  • To develop genetic and molecular tools for investigating dCNDP2 functions in D. melanogaster.
  • To create a null mutation and transgenic fly lines for dCNDP2 research.
  • To generate specific antibodies for dCNDP2 detection.

Main Methods:

  • CRISPR/Cas9-mediated homologous recombination to generate a dCNDP2 null mutation (∆dCNDP2).
  • Generation of transgenic fly lines expressing eGFP-tagged and non-tagged dCNDP2 under UAS control.
  • Production of polyclonal antibodies specific to dCNDP2.

Main Results:

  • ∆dCNDP2 mutants are viable, fertile, and morphologically normal.
  • Expression analysis revealed that only one of two predicted dCNDP2 isoforms is expressed.
  • dCNDP2 protein localizes to both cytoplasm and nucleus and associates with polytene chromosomes.

Conclusions:

  • dCNDP2 is not essential for viability in D. melanogaster under standard conditions.
  • The observed subcellular localization suggests potential roles in both cytoplasmic and nuclear processes.
  • The developed tools will facilitate future functional studies of the conserved CNDP2 protein in a model organism.
Keywords:
CG17337CN2CNDP2CPGLCRISPR/Cas9Drosophila melanogasterTumor suppressor gene

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