Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Platelet aggregating substance from mononuclear leukocytes.

M A Lazzari, M A Schattner, M Finiasz

    Prostaglandins, Leukotrienes, and Medicine
    |June 1, 1986
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Diet-microbiota interactions influence pregnancy success in females undergoing artificial insemination: insights from the vaginal microbiota and Mediterranean diet.

    Food & function·2026
    Same author

    Feasibility of a pilot randomized controlled trial on cranial remolding orthosis for severe deformational plagiocephaly.

    Canadian prosthetics & orthotics journal·2026
    Same author

    Perivascular wall tumour presenting as pastern mass in a Standardbred gelding.

    Australian veterinary journal·2023
    Same author

    Data quality validation of the Spanish Incisional Hernia Surgery Registry (EVEREG): pilot study.

    Hernia : the journal of hernias and abdominal wall surgery·2023
    Same author

    Evaluation of data quality in the Spanish EURECCA Esophagogastric Cancer Registry.

    European journal of surgical oncology : the journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·2021
    Same author

    Intraoperative electron beam radiotherapy and perioperative high-dose-rate brachytherapy in previously irradiated oligorecurrent gynecological cancer: clinical outcome analysis.

    Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·2021

    Human leukocytes stimulated with arachidonic acid (AA) induce platelet aggregation. This effect involves AA metabolism via cyclooxygenase and lipoxygenase pathways, suggesting novel platelet-activating substances.

    Area of Science:

    • Immunology
    • Biochemistry
    • Hematology

    Background:

    • Human peripheral blood mononuclear leukocytes (ML) can influence platelet function.
    • Arachidonic acid (AA) is a precursor to various signaling molecules involved in inflammation and hemostasis.

    Purpose of the Study:

    • To investigate the ability of AA-stimulated ML to induce platelet aggregation.
    • To elucidate the metabolic pathways involved in the generation of platelet-activating substances by ML.

    Main Methods:

    • Incubation of human peripheral blood mononuclear leukocytes (ML) with arachidonic acid (AA).
    • Assessment of platelet aggregation response (shape change, first and second wave aggregation).
    • Inhibition studies using aspirin, 5, 8, 11, 14 eicosatetraynoic acid (ETYA), and nordihydroguaiaretic acid (NDGA).

    Related Experiment Videos

    Main Results:

    • AA-stimulated ML induced significant platelet aggregation, characterized by shape change and biphasic aggregation.
    • Cell-free supernatants from AA-stimulated ML also induced platelet aggregation, indicating secreted mediators.
    • Aspirin, ETYA, and NDGA partially inhibited the aggregatory activity, suggesting involvement of cyclooxygenase and lipoxygenase pathways.
    • Complete cyclooxygenase integrity was essential for the second wave of aggregation, but not the first.

    Conclusions:

    • ML stimulated with AA produce platelet-activating substance(s) derived from AA metabolism.
    • These mediators likely involve both cyclooxygenase and lipoxygenase pathways.
    • The generated substance(s) are not hydroperoxy fatty acids or thromboxane A2, as they remain active for extended periods.