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Classification of wine grape biotypes according to their variety and sanitary condition by fingerprinting untargeted
Pasquale Crupi1, Marica Gasparro1, Angelo Raffaele Caputo1
1CREA-VE - Council for Agricultural Research and Economics, Research Centre for Viticulture and Enology, Turi (BA), Italy.
Abstract:
Grapes secondary metabolites content mainly depends on variety, but also on climate and cultural conditions, including sanitary status. This study aimed to use a metabolomic fingerprinting approach for grouping 72 wine grape biotypes, Negro amaro n. (N), Malvasia nera di Brindisi/Lecce n. (M), and Uva di Troia n. (U), on the basis of their cultivar and virological conditions. The skins were extracted and analysed by flow injection mass spectrometry; a one-way ANOVA/Principal Component Analysis (PCA) allowed to efficiently cluster the samples, recognizing M from N and U biotypes. Conversely, the clusterisation of the biotypes affected by different virus complexes was really more tough and a clear distinction among infected plants was not always observed. However, very interestingly, by applying ANOVA/PCA to the biotypes of each varieties, singularly, healthy biotypes were sharply separated in all the varieties and a relationship between anthocyanin compounds and Grapevine leafroll associated virus (GLRaV3) slightly appeared.
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