Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Bioavailability Study Design: Single Versus Multiple Dose Studies01:11

Bioavailability Study Design: Single Versus Multiple Dose Studies

227
Bioavailability studies are essential for understanding how a drug is absorbed, distributed, metabolized, and excreted in the body. These studies assess the extent and rate at which the active pharmaceutical agent becomes available at the site of action. The design of bioavailability studies can involve single-dose or multiple-dose regimens, each with distinct advantages and limitations.Single-dose studies are the preferred approach due to their simplicity and reduced drug exposure for...
227
Multiple Allele Traits01:49

Multiple Allele Traits

38.1K
The Concept of Multiple Allelism
38.1K
Multiple Regression01:25

Multiple Regression

3.9K
Multiple regression assesses a linear relationship between one response or dependent variable and two or more independent variables. It has many practical applications.
Farmers can use multiple regression to determine the crop yield based on more than one factor, such as water availability, fertilizer, soil properties, etc. Here, the crop yield is the response or dependent variable as it depends on the other independent variables. The analysis requires the construction of a scatter plot...
3.9K
Chromatin Immunoprecipitation- ChIP02:36

Chromatin Immunoprecipitation- ChIP

12.3K
Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
12.3K
Deformation of Member under Multiple Loadings01:11

Deformation of Member under Multiple Loadings

465
When a rod is made of different materials or has various cross-sections, it must be divided into parts that meet the necessary conditions for determining the deformation. These parts are each characterized by their internal force, cross-sectional area, length, and modulus of elasticity. These parameters are then used to compute the deformation of the entire rod.
In the case of a member with a variable cross-section, the strain is not constant but depends on the position. The deformation of an...
465
Multiple Voltage Sources01:25

Multiple Voltage Sources

1.8K
Generally, a single battery is not enough to power some devices. In such cases, batteries can be combined in two ways: in series or in parallel.
In series, the positive terminal of one battery is connected to the negative terminal of another battery. Hence, the voltage of each battery is added to give the net voltage, which is increased because each battery boosts the electrons that enter it. The same current flows through each battery because they are connected in series.
Batteries are...
1.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A decoy virus strategy using plant-produced SARS-CoV-2 virus-like-particles to inhibit infection and lung injury.

Journal of translational medicine·2026
Same author

A saliva-based surrogate associates with clinical outcome of oral potentially malignant disorders.

British journal of cancer·2026
Same author

Mapping cellular and ECM heterogeneity in pulmonary fibrosis - insights from recent spatiomics studies.

Cell biomaterials·2026
Same author

Two-Year Burden of Antibiotic Use for Prosthetic Joint Infection Following Total Knee Arthroplasty.

Surgical infections·2026
Same author

Analysis of positive surveillance cultures of flexible gastrointestinal endoscopes: a 6-year study in a tertiary medical centre in Taiwan.

The Journal of hospital infection·2026
Same author

Gelsemine induces apoptosis through a reactive oxygen species- and MAPK/JNK1/2-coregulated pathway in tongue squamous cell carcinoma cells.

Toxicon : official journal of the International Society on Toxinology·2026

Related Experiment Video

Updated: Jan 27, 2026

Establishing Single-Cell Based Co-Cultures in a Deterministic Manner with a Microfluidic Chip
07:05

Establishing Single-Cell Based Co-Cultures in a Deterministic Manner with a Microfluidic Chip

Published on: September 27, 2019

6.5K

Hydrodynamic shuttling for deterministic high-efficiency multiple single-cell capture in a microfluidic chip.

Cheng-Kun He1, Ya-Wen Chen, Ssu-Han Wang

  • 1Ph.D. Program in Tissue Engineering and Regenerative Medicine, National Chung Hsing University, Taichung, Taiwan.

Lab on a Chip
|March 20, 2019
PubMed
Summary
This summary is machine-generated.

A new hydrodynamic shuttling chip (HSC) enables precise single-cell co-culture for studying cellular interactions. This method significantly improves cell pairing efficiency, revealing cell-type-dependent motility in oral cancer research.

More Related Videos

A Microfluidic-based Hydrodynamic Trap for Single Particles
10:13

A Microfluidic-based Hydrodynamic Trap for Single Particles

Published on: January 21, 2011

17.2K
A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

15.5K

Related Experiment Videos

Last Updated: Jan 27, 2026

Establishing Single-Cell Based Co-Cultures in a Deterministic Manner with a Microfluidic Chip
07:05

Establishing Single-Cell Based Co-Cultures in a Deterministic Manner with a Microfluidic Chip

Published on: September 27, 2019

6.5K
A Microfluidic-based Hydrodynamic Trap for Single Particles
10:13

A Microfluidic-based Hydrodynamic Trap for Single Particles

Published on: January 21, 2011

17.2K
A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

15.5K

Area of Science:

  • Biotechnology
  • Cell Biology
  • Cancer Research

Background:

  • Cellular heterogeneity studies are crucial for disease treatment strategies, particularly in cancer.
  • In vitro co-culture experiments for studying individual live cell interactions are challenging to establish.
  • Understanding cell-cell interactions is key to advancing disease treatment.

Purpose of the Study:

  • To develop a novel microfluidic device for precise single-cell co-culture experiments.
  • To investigate the impact of cell-cell interactions on cell motility using a new co-culture method.
  • To overcome limitations of existing methods in studying live cell interactions.

Main Methods:

  • Development of a hydrodynamic shuttling chip (HSC) for deterministic single-cell capture.
  • Setting up multiple single-cell co-culture experiments within microfluidic chambers.
  • Microscopic observation of individual live cells during co-culture.
  • Demonstration using oral squamous cell carcinoma and lymphatic endothelial cells.

Main Results:

  • The HSC device achieved an eightfold higher single-cell pairing efficiency compared to probabilistic methods.
  • Successfully demonstrated triple single-cell cultures of cancer and endothelial cells.
  • Observed cell type-dependent motility influenced by cell-cell interactions.
  • Identified motility patterns not discernible in traditional co-culture experiments.

Conclusions:

  • The hydrodynamic shuttling chip is an effective tool for high-efficiency single-cell co-culture.
  • This technology facilitates the study of cell-cell interactions and their impact on cellular behavior.
  • The findings provide new insights into oral squamous cell carcinoma and lymphatic endothelial cell motility.