Related Experiment Video
Updated: Jan 27, 2026

Bergmeyer Glucose Quantification for Microbiological Samples
Published on: January 17, 2025
A facile SERS strategy for quantitative analysis of trace glucose coupling glucose oxidase and nanosilver catalytic
Dongmei Yao1, Chongning Li2, Aihui Liang2
1Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Guangxi Normal University, Ministry of Education, Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin 541004, China; College of Chemistry and Biology Engineering, Hechi University, Yizhou 546300, China.
Abstract:
Highly stable, SERS active and catalytic nanosilver sol (AgNP) was synthesized under the exposure of light wave, using AgNO3 as precursor and sodium citrate as reducer. Under the conditions of pH 7.0 NaH2PO4-Na2HPO4 buffer solution (PBS), the glucose can be catalyzed by glucose oxidase to produce H2O2 specifically. Based on the nanocatalyst and SERS substrate of AgNP, H2O2 can oxidize the 3,3',5,5'-tetramethylbenzidine (TMB) quickly to form a blue oxidation product (TMBox) that induced the AgNPs aggregation, which exhibited a strong SERS signal at 1606 cm-1. As the concentration of glucose increases, the TMBox molecular probes and AgNPs aggregation increase, and the intensity of SERS peak at 1606 cm-1 increase linearly. Thus, a new SERS strategy for quantitative analysis of 0.33-6.67 μmol/L glucose was developed, with a detection limit of 0.035 μmol/L, coupled the catalysis of nanosilver with glucose oxidase, and label-free molecular probe of TMBox.
More Related Videos
Related Concept Videos
Glucose Homeostasis: Regulation of Blood Glucose
During fasting, when blood glucose levels are low, the pancreas secretes glucagon. it...
Glucose Transporters
Facilitated diffusion-glucose transporters (GLUTs) are encoded by the solute-linked carrier (SLC) family 2, subfamily A gene family, or SLC2A. The 14 GLUT protein members are distributed into three classes:
Glucose Absorption Into the Small Intestine
Hormones Regulating Blood Glucose
In addition to accelerating glucose uptake and utilization, insulin has...
Glucose Homeostasis: Pancreatic Islets and Insulin Secretion
Insulin and C-peptide are...
Quantitative Analysis
In quantitative analysis, two key measurements are made: the sample quantity and a property proportional to the amount of the analyte (the substance being analyzed). This forms the basis of the...

