Antibacterial and cytocompatibility study of modified Ti6Al4V surfaces through thermal annealing
Deepak Patil1, Mishi Kaushal Wasson2, S Aravindan1
1Department of Mechanical Engineering, Indian Institute of Technology Delhi, New Delhi 110016, India.
Abstract:
Silver coating of different thicknesses ranging from 5 to 20 nm was deposited on the Ti6Al4V substrate using DC sputtering followed by thermal annealing at 750 °C for 15 min in an ambient environment. The surface topography and elemental composition of annealed samples were analyzed using different characterization techniques. The silver ions (Ag+) concentration released from the modified titanium surface was calculated through inductive coupled plasma mass spectroscopy (ICP-MS). The plate counting method was used to quantify the bacteria-killing potential of modified titanium surface against Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), Fluoroquinolones-resistant Salmonella typhi (FRST) and Methicillin-resistant Staphylococcus aureus (MRSA) bacteria. The cell membrane integrity study of E. coli and S. aureus bacterium was done qualitatively using scanning electron microscopy and further confirmed with fluorescence microscopy. Due to thermal annealing, polygonal shaped oxide nanoparticles were formed on the titanium substrate. Moreover, the surface topography of modified titanium surface changes with the thickness of the silver film. In order to check the cytotoxic effect of modified titanium surface, mouse fibroblast cells (NIH3T3) were used for 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide (MTT) assay. The limited (<35 ppb) Ag+ ion release was noticed for 15 nm silver film which has shown the good bactericidal property and significant growth of fibroblast cells. This study proposes a simple and efficient method to enhance the antibacterial property of Ti6Al4V surfaces to avoid implant-related infection.
Related Concept Videos
Thermal expansion and Thermal stress: Problem Solving
To solve the problem, first, identify the known and unknown quantities. The initial length (L) of the bridge is 1275 m, the coefficient of linear expansion (α) for steel is 12 x 10-6/°C, and the change in temperature (ΔT) is 55...
Thermal Strain
Thermal Expansion
Thermal Stress
Temperature and Thermal Equilibrium
The concept of temperature has evolved from the common concepts of hot and cold. The scientific definition of temperature explains more than just our sense of hot and cold. Temperature is operationally defined as the quantity measured with a thermometer. Furthermore, temperature is...
Surface Tension, Capillary Action, and Viscosity
The various IMFs between identical molecules of a substance are examples of cohesive forces. The molecules within a liquid are surrounded by other molecules and are attracted equally in all directions by the cohesive forces within the liquid. However, the molecules on the surface of a liquid are attracted only by about one-half as many molecules. Because of the unbalanced molecular attractions on the surface molecules, liquids contract to form a shape that minimizes the number...


