[3-component nutrient medium for the nisin producer Streptococcus lactis strain MGU]

The methods of mathematical desing of experiments such as Box and full factorial design were used in the study. As a result a three-component medium including 1-2 per cent of mollases, 10-20 mg% of yeast autolysate and 1-2 per cent of KH2PO4 was recommended for biosynthesis of nisin.