Interfacial Assembly of Signal Amplified Multienzymes and Biorecognized Antibody into Proteinosome for an...

Enzyme as signal tag has been widely employed in colorimetric immunoassays for decades. Nevertheless, it remains a great challenge to substantially improve the detection sensitivity of enzyme-based immunoassays, which inhibits further critical applications. To circumvent this confinement, a multifunctional self-assembled proteinosome based on the integration of signal amplification elements (enzyme) and biorecognition unit (antibody) is proposed for fabricating an immunoassay strategy with significantly enhanced sensitivity. Owing to the self-assembly technique, this proteinosome not only efficiently loads abundant enzymes to possess high catalytic activity, but also enhances enzymatic stability and maintains recognition ability of antibody. Using imidacloprid as a model target, the proteinosome-based immunoassay reaches a limit of detection down to the picogram mL^-1 level, which is 150-fold lower than that of conventional enzyme-linked immunosorbent assay. This method provides a versatile approach for constructing spherical proteinosome as a recognizer and amplifier for profiling a broad range of target antigen.