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Author Spotlight: Advancing the Analysis of Plasma Extracellular Vesicle Proteome for Cardiovascular Biomarker Studies
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Proteomic Analysis of Human Plasma during Intermittent Fasting
Dylan J Harney1, Amy T Hutchison2, Luke Hatchwell1
1Charles Perkins Centre, School of Life and Environmental Sciences , University of Sydney , Sydney , NSW 2006 , Australia.
Journal of Proteome Research
|March 21, 2019
Summary
Intermittent fasting (IF) shows promise for human health, improving lipid metabolism by altering key apolipoproteins. This study optimized proteomics for analyzing clinical plasma samples, revealing IF
Area of Science:
- Proteomics
- Human Health
- Metabolic Disease
Background:
- Intermittent fasting (IF) shows benefits in model organisms but human data is limited.
- Analyzing human plasma proteomics is challenging due to extensive sample preparation.
- Efficient sample processing is crucial for statistically significant results in clinical trials.
Purpose of the Study:
- To optimize a device (Spin96) for efficient human plasma sample preparation for LC-MS/MS.
- To investigate the effects of 8-week intermittent fasting on human plasma proteomes.
- To identify protein abundance changes and correlations with metabolic markers.
Main Methods:
- Development and application of the Spin96 device for StageTip-based plasma sample preparation.
- Liquid chromatography-tandem mass spectrometry (LC-MS/MS) for proteomic analysis.
- Utilized a novel human protein variant database for variant detection.
Main Results:
- Identified significant changes in apolipoprotein levels (increased APOA4, decreased APOC2, APOC3) after 8 weeks of IF.
- Observed a significant decrease in plasma triglycerides correlating with apolipoprotein changes.
- Demonstrated the ability to detect clinically relevant protein variants from both alleles.
Conclusions:
- Intermittent fasting positively impacts lipid metabolism in humans, potentially via HDL modulation.
- Accessible proteomics workflows enhance the analysis of clinical samples for biological insights.
- This study provides a foundation for further research into IF's health benefits using advanced proteomics.
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