Open-Top Light-Sheet Microscopy Image Atlas of Prostate Core Needle Biopsies.

Area of Science:

• Pathology
• Microscopy
• Biotechnology

Background:

• Ex vivo microscopy allows tissue examination without embedding or slide preparation.
• Light-sheet microscopy enables rapid 3D imaging of biological samples.
• An open-top light-sheet (OTLS) microscope was developed for pathology applications.

Purpose of the Study:

• To create an image atlas of prostate core needle biopsies using OTLS microscopy.
• To evaluate the utility of OTLS microscopy in pathology.

Main Methods:

• Nine prostate core needle biopsies were obtained from radical prostatectomy specimens.
• Biopsies were fixed, dehydrated, stained (TO-PRO3, eosin), optically cleared, and imaged with OTLS microscopy.
• Subsequent processing included paraffin embedding, sectioning, and standard hematoxylin-eosin and immunohistochemical staining.

Main Results:

• OTLS microscopy images demonstrated high fidelity with traditional light microscopy for benign and neoplastic structures.
• OTLS imaging did not negatively impact subsequent hematoxylin-eosin or immunohistochemical staining.
• 3D histology from OTLS microscopy revealed novel structural insights, such as contiguous glandular morphologies and continuity between poorly and well-formed glands.

Conclusions:

• Three-dimensional OTLS microscopy images closely resemble traditional histology while providing valuable structural information.
• OTLS microscopy is a promising tool for pathology, offering enhanced visualization of tissue architecture.
• Further research is necessary to explore OTLS microscopy across diverse tissues and deepen the understanding of 3D histology.