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The overexpression of lncRNA H19 as a diagnostic marker for coronary artery disease
Gang Xiong1, Xuejun Jiang2, Tao Song2,3
1Department of Cardiology, Wuhan Asia Heart Hospital, Wuhan, China.
Insights
Long non-coding RNA H19 is upregulated in coronary artery disease (CAD) patients and can accurately predict CAD. H19 may also play a role in CAD development by increasing TGF-β1 expression.
Area of Science:
- Biochemistry
- Molecular Biology
- Cardiovascular Research
Background:
- Coronary artery disease (CAD) poses a significant global health challenge.
- Identifying reliable biomarkers for early diagnosis and understanding disease mechanisms are crucial for effective management.
Purpose of the Study:
- To evaluate the diagnostic potential of long non-coding RNA H19 (lncRNA H19) in coronary artery disease (CAD).
- To investigate the underlying mechanisms of lncRNA H19 in CAD pathogenesis.
Main Methods:
- Quantitative reverse transcription polymerase chain reaction (qRT-PCR) to measure lncRNA H19 expression in serum samples from CAD patients and healthy controls.
- Receiver operating characteristic (ROC) curve analysis to assess the diagnostic accuracy of lncRNA H19.
- Enzyme-linked immunosorbent assay (ELISA) to quantify serum TGF-β1 levels.
- Western blot analysis to determine the effect of H19 overexpression on TGF-β1 expression in human primary coronary artery endothelial cells (HCAECs).
Main Results:
- lncRNA H19 expression was significantly higher in CAD patients compared to healthy individuals and patients with other cardiovascular diseases.
- ROC curve analysis demonstrated high diagnostic accuracy for lncRNA H19 in identifying CAD.
- H19 expression showed a positive correlation with serum TGF-β1 levels.
- Overexpression of H19 in HCAECs led to a significant increase in TGF-β1 protein levels.
Conclusions:
- lncRNA H19 is a potential diagnostic biomarker for CAD.
- H19 overexpression contributes to CAD pathogenesis by upregulating TGF-β1 expression, suggesting a novel therapeutic target.
Objective:
Our study aimed to investigate the diagnostic value of lncRNA H19 for coronary artery disease (CAD) and to explore its possible mechanisms.
Methods:
A total of 30 CAD patients and 30 healthy individuals, as well as patients with different cardiovascular diseases, were included in this study. Blood was drawn from each participant to prepare serum samples, and the expression of lncRNA H19 was detected using qRT-PCR. The ROC curve analysis was used to analyze the diagnostic value of H19 for CAD. The effects of patients' basic information and lifestyle on H19 expression were analyzed. The plasma level of TGF-β1 was measured by ELISA. The H19 overexpression in the human primary coronary artery endothelial cell (HCAEC) line was constructed, and the effects of H19 overexpression on the TGF-β1 expression were analyzed using Western blot. The results of H19 expression were specifically upregulated in patients with CAD but not in healthy individuals and patients with other types of cardiovascular diseases. The ROC curve analysis showed that the H19 expression level could be used to predict CAD accurately. Gender, age, and patients' lifestyle had no significant effects on H19 expression, but H19 expression was higher in patients with a longer course of disease in comparison with the controls. H19 expression was positively correlated with the serum level of TGF-β1, and H19 overexpression significantly increased TGF-β1 protein level in HCAEC.
Conclusion:
H19 overexpression participates in the pathogenesis of CAD by increasing the expression level of TGF-β1, and H19 expression level may serve as a diagnostic marker for CAD.
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