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Expression of M30 and M65 in celiac disease. Analytical cross-sectional study
Evrim Kahramanoğlu Aksoy1, Gülçin Güler Şimşek2, Murat Torgutalp3
1MD. Gastroenterologist, Department of Gastroenterology, Keçiören Training and Research Hospital, Ankara, Turkey.
Background:
The role of villous atrophy in apoptosis, a distinctive feature of celiac disease, is a matter of controversy. The aim of this study was to determine the apoptosis rate through immunohistochemical staining for M30 and M65 in celiac disease cases.
Design And Setting:
Analytical cross-sectional study in a tertiary-level center.
Methods:
Duodenal biopsies from 28 treatment-naive patients with celiac disease, 16 patients with potential celiac disease, 10 patients with a gluten-free diet and 8 controls were subjected to immunohistochemical staining for the end-apoptotic marker M30 and the total cell death marker M65. H-scores were compared. Several laboratory parameters were recorded concomitantly, and at the one-year follow-up for celiac disease and potential celiac disease patients.
Results:
There was a significant difference in H-score for M30 expression between the celiac disease, potential celiac disease and gluten-free diet groups (P = 0.009). There was no significant difference in H-score for M65 expression. There was a positive correlation between the H-score for M30 expression and the anti-tissue transglutaminase immunoglobulin A (anti-tTgIgA) and anti-tissue transglutaminase immunoglobulin G (anti-tTgIgG) levels (R = 0.285, P = 0.036; and R = 0.307, P = 0.024, respectively); and between the H-score for M65 expression and the anti-tTgIgA and anti-tTgIgG levels (R = 0.265, P = 0.053; and R=0.314, P = 0.021, respectively). There was no difference between celiac disease and potential celiac disease patients regarding the laboratory parameters selected.
Conclusion:
The rates of apoptosis and nutritional deficiencies in patients with potential celiac disease were similar to those in patients with celiac disease.
Insights
Apoptosis rates, measured by M30 staining, were significantly different in celiac disease and potential celiac disease patients compared to controls. These apoptosis rates correlated with celiac disease antibody levels.
Area of Science:
- Gastroenterology
- Immunology
- Cell Biology
Background:
- Villous atrophy in celiac disease is linked to apoptosis, but its exact role remains debated.
- This study investigates apoptosis rates in celiac disease using specific markers.
Purpose of the Study:
- To determine and compare apoptosis rates in celiac disease, potential celiac disease, gluten-free diet groups, and controls.
- To correlate apoptosis markers with serological markers of celiac disease.
Main Methods:
- An analytical cross-sectional study was conducted.
- Duodenal biopsies from 28 celiac disease, 16 potential celiac disease, 10 gluten-free diet, and 8 control patients were analyzed using M30 and M65 immunohistochemical staining.
- Laboratory parameters and one-year follow-up data were collected.
Main Results:
- M30 expression (apoptosis) differed significantly across celiac disease, potential celiac disease, and gluten-free diet groups.
- M65 expression (total cell death) showed no significant differences.
- M30 and M65 expression correlated positively with anti-tissue transglutaminase (tTg) IgA and IgG levels.
Conclusions:
- Apoptosis rates in potential celiac disease patients are similar to those in celiac disease patients.
- Nutritional deficiencies were also comparable between potential celiac disease and celiac disease groups.
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