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Mitogenic factor from chronically infected guinea pigs
Abstract:
A lymphokine produced by antigen stimulated lymphocytes, induces blastogenesis in cultures of lymphocytes which are not sensitive to the specific antigen. The in vitro production of this factor (MF) was accomplished utilizing peritoneal exudate (PE) cells from Coccidioides immitis infected guinea pigs. Production of MF by lymphoid cultures paralleled skin test reactivity of the donor animal. Removal of adherent cells from the PE population did not decrease the production of MF; conversely, a more significant production of MF was effected by the adherent cell depleted populations. Maximal production of MF was achieved at non-adherent cell concentrations from 4 X 10(6) to 8 X 10(6) cells/ml. Cell concentrations below 4 X 10(6)/ml produced material which inhibited DNA synthesis in test cultures. MF was separated from the inhibitory substance(s) by column chromatography of the crude preparations on Sephadex G-75. Inhibitor(s) eluted in the void volume (VO), and the MF eluted in an effluent volume (Ve) which was greater than the total bed volume (Vt) suggesting that MF is adsorbed by Sephadex beads.
Insights
Researchers identified a lymphokine (MF) from Coccidioides immitis-infected guinea pigs that induces lymphocyte blastogenesis. This factor
Area of Science:
- Immunology
- Cell Biology
Background:
- Lymphokines are crucial mediators in immune responses.
- Understanding lymphokine production is key to deciphering cellular immunity.
Purpose of the Study:
- To characterize a novel lymphokine (MF) produced by antigen-stimulated lymphocytes.
- To investigate the in vitro production and properties of MF.
Main Methods:
- Utilized peritoneal exudate (PE) cells from Coccidioides immitis-infected guinea pigs for in vitro MF production.
- Assessed MF production in relation to lymphoid cell concentrations and adherent cell presence.
- Employed Sephadex G-75 column chromatography for MF separation.
Main Results:
- MF production correlated with donor animal skin test reactivity.
- Adherent cell-depleted populations showed enhanced MF production.
- Maximal MF production occurred at 4-8 X 10^6 non-adherent cells/ml.
- Suboptimal cell concentrations yielded DNA synthesis inhibitors.
Conclusions:
- MF induces blastogenesis in non-specifically sensitive lymphocytes.
- MF production is influenced by adherent cell populations and cell density.
- MF exhibits properties suggesting adsorption to Sephadex beads.