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Mitogenic factor from chronically infected guinea pigs.

J F Carlquist, S Marcus

    Immunological Communications
    |January 1, 1978
    PubMed
    Summary
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    Researchers identified a lymphokine (MF) from Coccidioides immitis-infected guinea pigs that induces lymphocyte blastogenesis. This factor

    Area of Science:

    • Immunology
    • Cell Biology

    Background:

    • Lymphokines are crucial mediators in immune responses.
    • Understanding lymphokine production is key to deciphering cellular immunity.

    Purpose of the Study:

    • To characterize a novel lymphokine (MF) produced by antigen-stimulated lymphocytes.
    • To investigate the in vitro production and properties of MF.

    Main Methods:

    • Utilized peritoneal exudate (PE) cells from Coccidioides immitis-infected guinea pigs for in vitro MF production.
    • Assessed MF production in relation to lymphoid cell concentrations and adherent cell presence.
    • Employed Sephadex G-75 column chromatography for MF separation.

    Main Results:

    • MF production correlated with donor animal skin test reactivity.

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  • Adherent cell-depleted populations showed enhanced MF production.
  • Maximal MF production occurred at 4-8 X 10^6 non-adherent cells/ml.
  • Suboptimal cell concentrations yielded DNA synthesis inhibitors.

    • Conclusions:

    • MF induces blastogenesis in non-specifically sensitive lymphocytes.
    • MF production is influenced by adherent cell populations and cell density.
    • MF exhibits properties suggesting adsorption to Sephadex beads.