The primary structure of threonine tRNA (anticodon I-G-U) from bovine liver

The primary structure of threonine tRNA (anticodon I-G-U) from bovine liver has been determined using 32P postlabeling methods. Its nucleotide sequence is pG-G-C-C-C-U-G-U-m1G-m2G-C-U-A-G-C-D-G-G-D-C-A-A-A-G-C-m2(2)G-C-C- U-G-U-m3 C-U-I-G-U-t6A-A-A-C-A-G-G-A-G-A-D-m5C-C-U-G-G-G-U-psi-C-G-m1 A-A-U-C-C-C-A-G-C-G-G-G-G-C-m5C-U-C-C-A. The anticodon I-G-U, according to the wobble hypothesis, is expected to recognize A-C-U, A-C-C and A-C-A, 3 out of the 4 codons for threonine. However, if the original wobble hypothesis, as it has been shown recently for several yeasts and a fungus, is only restricted to U and C in the ox, it would recognize only 2 codons: A-C-C and A-C-U.