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Updated: Jan 27, 2026

Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells
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Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells

Published on: February 25, 2007

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Nidogen-1 Contributes to the Interaction Network Involved in Pro-B Cell Retention in the Peri-sinusoidal

Marielle Balzano1, Maria De Grandis2, Thien-Phong Vu Manh3

  • 1Aix-Marseille University, CNRS, INSERM, Institut Paoli-Calmettes, CRCM, Marseille, France; Aix-Marseille University, CNRS, INSERM, CIML, Marseille, France.

Cell Reports
|March 21, 2019
PubMed

Insights

Stromal cells in the bone marrow support both hematopoietic stem cells and developing B cells. Nidogen-1 is crucial for retaining pro-B cells in this niche, which is conserved in humans.

Area of Science:

  • Hematology
  • Stem Cell Biology
  • Immunology

Background:

  • Hematopoietic stem cells (HSCs) and B cell differentiation rely on bone marrow stromal cells, specifically CXCL12 and IL-7.
  • Peri-sinusoidal stromal (PSS) cells are primary IL-7 sources, but their role in supporting pro-B cells is not fully understood.

Purpose of the Study:

  • To characterize pro-B cell supporting stromal cells.
  • To identify the molecular interactions governing pro-B cell retention within the niche.

Main Methods:

  • Characterization of stromal cells and their interactions with pro-B cells.
  • Bioinformatic interactome analysis.
  • Validation in Nidogen-1 knockout mice.

Main Results:

  • Pro-B cells preferentially interact with PSS cells expressing HSC and B cell niche genes.
  • Pro-B cells and HSCs coexist within the same niche.
  • Nidogen-1 was identified as essential for pro-B cell retention in the peri-sinusoidal niche.
  • Human pro-B cells and progenitors are found near similar IL-7+ stromal cells.

Conclusions:

  • A multispecific niche supporting early progenitors and committed hematopoietic lineages exists in both mice and humans.
  • Nidogen-1 plays a critical role in maintaining the pro-B cell niche.

Keywords:
B cell developmentbone marrowhematopoietic stem cellinteraction networkstromal cell niche

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