Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity07:52

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity

7.0K
Here we describe a rapid and direct in vivo CRISPR/Cas9 screening methodology using ultrasound-guided in utero embryonic lentiviral injections to simultaneously assess functions of several genes in the skin and oral cavity of immunocompetent...
7.0K
In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression08:54

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

7.5K
This protocol outlines the steps needed to generate a model system in which the transcription of an endogenous gene of interest can be conditionally controlled in live animals or cells using enhanced lac repressor and/or tet activator...
7.5K
A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer11:53

A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer

18.9K
This protocol describes the steps for cloning multiple single guide RNAs into one guide RNA concatemer vector, which is of particular use in creating multi-gene knockouts using CRISPR/Cas9 technology. The generation of double knockouts in intestinal organoids is shown as a possible application of this...
18.9K
Generation of Genetically Modified Mice through the Microinjection of Oocytes10:19

Generation of Genetically Modified Mice through the Microinjection of Oocytes

21.6K
The microinjection of mouse oocytes is commonly used for both classic transgenesis (i.e., the random integration of transgenes) and CRISPR-mediated gene targeting. This protocol reviews the latest developments in microinjection, with a particular emphasis on quality control and genotyping...
21.6K
DNA Vector-based RNA Interference to Study Gene Function in Cancer13:10

DNA Vector-based RNA Interference to Study Gene Function in Cancer

21.0K
RNA interference (RNAi) possesses many advantages over gene knockout and has been broadly used as a tool in gene functional studies. The invention of DNA vector-based RNAi technology has made long term and inducible gene knockdown possible, and also increased the feasibility of gene silencing in...
21.0K
Quantitative and Automated High-throughput Genome-wide RNAi Screens in C. elegans10:58

Quantitative and Automated High-throughput Genome-wide RNAi Screens in C. elegans

18.2K
We describe a protocol using C. elegans and RNAi feeding libraries that allows automated measurement of multiple parameters such as fluorescence, size and opacity of individual worms in a population. We give one example of a screen to identify genes involved in anti-fungal innate immunity in C.
18.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Intersegmental transfers drive target search in an RNA-targeting CRISPR system.

bioRxiv : the preprint server for biology·2026
Same author

Modulation of Oncogenic KRAS Signaling by Branched Actin-driven Cell Membrane Protrusions.

Research square·2026
Same author

Volumetric Cyclic Immunofluorescence for 3D Spatial Profiling of Immune Structures in Human FFPE Tissue.

bioRxiv : the preprint server for biology·2026
Same author

Multimodal control of Cas13d activity through domain insertion at an allosteric hotspot.

Nature communications·2026
Same author

Towards deployable CRISPR-based nucleic acid detection.

Progress in biomedical engineering (Bristol, England)·2026
Same author

A National Prospective Multicenter Comparison of Risk Scores Against Clinical Judgement for the Prediction of Safe Discharge From the Emergency Department in Patients Presenting With Lower Gastrointestinal Bleeding.

Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association·2026
Same journal

Inside the new political screening that's stalling NIH grants.

Nature·2026
Same journal

Europe's record heatwave: does the continent have a new climate?

Nature·2026
Same journal

Daily briefing: Humans and great apes giggle in the same rhythms.

Nature·2026
Same journal

The surprising career parallels between footballers and researchers.

Nature·2026
Same journal

I study World Cup penalty shoot-outs: they say a lot about the psychology of performance under pressure.

Nature·2026
Same journal

CRISPR's next act: the companies editing the epigenome to treat disease.

Nature·2026
See all related articles

Related Experiment Video

Updated: Jan 14, 2026

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity
07:52

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity

Published on: November 2, 2020

7.0K

Diverse and robust molecular algorithms using reprogrammable DNA self-assembly.

Damien Woods1,2,3, David Doty4,5, Cameron Myhrvold6,7

  • 1California Institute of Technology, Pasadena, CA, USA. damien.woods@mu.ie.

Nature
|March 22, 2019
PubMed
Summary
This summary is machine-generated.

Researchers developed a large DNA tile set enabling molecular self-assembly to execute complex algorithms. This breakthrough paves the way for programmable matter and advanced molecular machines.

More Related Videos

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
08:54

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

Published on: March 29, 2019

7.5K
A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer
11:53

A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer

Published on: July 12, 2017

18.9K

Related Experiment Videos

Last Updated: Jan 14, 2026

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity
07:52

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity

Published on: November 2, 2020

7.0K
In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
08:54

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

Published on: March 29, 2019

7.5K
A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer
11:53

A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer

Published on: July 12, 2017

18.9K

Area of Science:

  • Molecular nanotechnology
  • Information-based chemistry
  • Algorithmic self-assembly

Background:

  • Molecular biology demonstrates chemical systems' capacity for information storage and processing.
  • Algorithmic behavior can be embedded in molecular self-assembly, experimentally shown with DNA nanotechnology.
  • A complexity threshold exists for information technologies, previously unaddressed in DNA self-assembly.

Purpose of the Study:

  • To investigate if DNA self-assembly can exceed the complexity threshold for programmable systems.
  • To design and validate a DNA tile set capable of executing diverse algorithms.

Main Methods:

  • Designed a DNA tile set comprising 355 single-stranded tiles.
  • Experimentally validated the tile set by reprogramming it to implement various 6-bit algorithms.
  • Constructed 21 distinct DNA circuits to execute algorithms with high fidelity.

Main Results:

  • Successfully reprogrammed the DNA tile set to implement a wide range of 6-bit algorithms.
  • Demonstrated execution of algorithms including copying, sorting, pattern generation, and computation.
  • Achieved a low per-tile error rate of less than 1 in 3,000.

Conclusions:

  • Molecular self-assembly can serve as a reliable component in programmable chemical systems.
  • The developed system suggests the feasibility of exceeding the complexity threshold in molecular computation.
  • Enables a new creative space for molecular programmers to develop advanced molecular machines.