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The correlation between AMH assays differs depending on actual AMH levels.
Å Magnusson1,2, G Oleröd3, A Thurin-Kjellberg1,2
1Department of Obstetrics, Gynaecology and Reproductive Medicine, Sahlgrenska University Hospital, SE-413 45 Gothenburg, Sweden.
Human Reproduction Open
|March 22, 2019
Summary
Serum anti-Müllerian hormone (AMH) levels differ significantly between laboratory assays, especially at low values. This impacts ovarian reserve assessment and fertility treatment decisions, highlighting the need for assay standardization.
Area of Science:
- Reproductive endocrinology
- Clinical chemistry
- Biomarker validation
Background:
- Anti-Müllerian hormone (AMH) is a key biomarker for ovarian response and fertility treatment decisions.
- Variations in AMH assays, reference values, and sample stability complicate clinical interpretation.
- Standardization is crucial for reliable AMH assessment in clinical practice.
Purpose of the Study:
- To evaluate the correlation of serum AMH levels between two commonly used laboratory assays.
- To identify potential discrepancies in AMH measurements that could affect clinical decisions.
Main Methods:
- An observational study compared AMH levels in 269 serum samples from infertile women.
- Serum AMH was analyzed using the Beckman Coulter Gen II ELISA original assay and the Modified Beckman Coulter Gen II ELISA (Premix method).
- Samples were handled identically and analyzed in parallel using both assays.
Main Results:
- The Premix method showed a mean of 18% higher AMH values compared to the Gen II original assay.
- For low AMH levels, the Premix method yielded over 40% higher values.
- Significant differences were observed, particularly impacting the interpretation of low AMH values.
Conclusions:
- Considerable differences in serum AMH levels exist between the two assays, especially for low values.
- These discrepancies can lead to misclassification of ovarian reserve and affect fertility counseling.
- There is an urgent need for international standards for AMH assay interpretation to ensure clinical consistency.
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