In vitro cellular viability studies on a concentrated surfactant-based wound dressing

Area of Science:

• Biomaterials Science
• Cell Biology
• Wound Healing Research

Background:

• Evaluating the biocompatibility and efficacy of topical wound care agents is crucial.
• Surfactant gels are increasingly explored for wound management.
• Assessing cytotoxicity and pro-healing properties of novel formulations is essential.

Purpose of the Study:

• To compare the biocompatibility and wound healing potential of two concentrated surfactant gels.
• To investigate the cytotoxic effects on L929 and human dermal fibroblasts (HDFa).
• To evaluate the impact on cell migration, wound closure, and membrane integrity.

Main Methods:

• Cytotoxicity was assessed using direct contact, extraction, and cell insert assays.
• An in vitro wound model was used to evaluate cell migration and closure.
• A needle-scratched membrane disruption model assessed membrane stabilization and resealing.

Main Results:

• The antimicrobial-preserved surfactant gel showed no toxicity to L929 and HDFa cells.
• The silver sulfadiazine gel exhibited cytotoxicity towards both cell types.
• The antimicrobial gel enhanced cell migration, accelerated wound closure, reduced necrosis, and improved membrane integrity.

Conclusions:

• The concentrated surfactant gel preserved with antimicrobials is non-cytotoxic and accelerates wound healing.
• This gel enhances cell mobility, stabilizes plasma membranes, and promotes wound closure.
• It offers a promising alternative for wound management compared to silver sulfadiazine formulations.