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Updated: Jan 27, 2026

Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor LATS Biosensor
07:16

Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor LATS Biosensor

Published on: September 13, 2018

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LATS2 inhibits cell proliferation and metastasis through the Hippo signaling pathway in glioma

Chengyong Guo1, Chaohui Liang1, Jipeng Yang1

  • 1Department of Neurosurgery, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei 050000, P.R. China.

Oncology Reports
|March 22, 2019
PubMed

Insights

Large tumor suppressor kinase 2 (LATS2) acts as a tumor suppressor in glioma. Downregulation of LATS2 inhibits cell proliferation, migration, and invasion, suggesting its role in glioma progression.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • The Hippo pathway is crucial for organ size control.
  • Large tumor suppressor kinase 2 (LATS2) is a core kinase in the Hippo pathway.
  • The role of LATS2 in glioma progression is not fully understood.

Purpose of the Study:

  • To investigate the function of LATS2 in glioma.
  • To determine the expression levels of LATS2 in glioma tissues and cells.
  • To elucidate the underlying molecular mechanisms of LATS2 in regulating glioma cell behaviors.

Main Methods:

  • Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and immunohistochemistry were used to assess LATS2 expression.
  • Cell Counting Kit-8, scratch wound healing, and Transwell assays were performed to evaluate cell proliferation, migration, and invasion.
  • Western blot analysis was employed to detect the expression of key signaling molecules.

Main Results:

  • LATS2 expression was significantly downregulated in glioma tissues and cells compared to normal tissues.
  • Overexpression of LATS2 in U-372 MG glioma cells suppressed cell proliferation, migration, and invasion.
  • LATS2 overexpression led to increased levels of phosphorylated yes-associated protein (p-YAP) and phosphorylated tafazzin (p-TAZ).

Conclusions:

  • LATS2 functions as a tumor suppressor in glioma.
  • Downregulation of LATS2 contributes to glioma progression.
  • Targeting LATS2 may represent a potential therapeutic strategy for glioma treatment.

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