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Peptidomic analysis of zebrafish embryos exposed to polychlorinated biphenyls and their impact on eye development

Yue Jiang1, Shuchun Zhang1, Xin Zhang2

  • 1Department of Pediatrics, Nanjing Medical University, Nanjing 210004, China.

Ecotoxicology and Environmental Safety
|March 22, 2019
PubMed

Insights

Polychlorinated biphenyls (PCBs) exposure disrupts normal eye development by altering peptide expression in zebrafish. This study identifies specific peptides linked to ocular dysplasia, offering potential biomarkers for treatment and understanding pollutant effects.

Area of Science:

  • Environmental Toxicology
  • Developmental Biology
  • Proteomics

Background:

  • Polychlorinated biphenyls (PCBs) are persistent organic pollutants linked to abnormal childhood eye development.
  • The specific role of peptides in PCB-induced ocular dysplasia remains largely uncharacterized.

Purpose of the Study:

  • To investigate the impact of PCB exposure on peptide expression during ocular system development.
  • To identify potential peptide biomarkers for PCB-induced ocular dysplasia.

Main Methods:

  • Utilized liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) to analyze peptide expression in normal and PCB-exposed zebrafish embryos.
  • Applied Ingenuity Pathway Analysis (IPA) and Gene Ontology (GO) analysis to explore the function of differentially expressed peptides.

Main Results:

  • Analyzed 7900 peptides, identifying 90 differentially expressed peptides (29 up-regulated, 61 down-regulated) in PCB-exposed zebrafish.
  • Identified 18 peptides associated with optic nerve and ocular system development in the PCB-exposed group.
  • Found 10 peptides located within the functional domains of their precursor proteins.

Conclusions:

  • Specific peptides are significantly altered by PCB exposure, impacting ocular development.
  • These differentially expressed peptides serve as potential biomarkers for diagnosing and potentially treating PCB-induced ocular dysplasia.
  • The findings contribute to understanding the molecular mechanisms underlying abnormal eye development caused by environmental pollutants.

Keywords:
LC-MS/MSOcular developmentPeptidePolychlorinated biphenylsZebrafish

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