New strategy for the gene mutation identification using surface enhanced Raman spectroscopy (SERS).

Area of Science:

• Nanotechnology
• Molecular Biology
• Spectroscopy

Background:

• Early and accurate diagnosis of specific DNA mutations is crucial for effective cancer treatment.
• Rapid confirmation of clinical findings is vital for immediate treatment decisions.
• The BRAF c.1799T>A; p. V600E mutation is a key target in several cancers.

Purpose of the Study:

• To develop a new strategy for identifying the BRAF V600E gene mutation.
• To create a highly sensitive and reproducible DNA biosensor.
• To demonstrate the utility of the biosensor in analyzing clinical samples.

Main Methods:

• Utilized a novel SERS substrate: photo-etched Gallium Nitride (GaN) covered with gold.
• Employed surface-enhanced Raman scattering (SERS) spectroscopy for detection.
• Detection based on conformation change of alkanethiol linker during DNA hybridization.

Main Results:

• Achieved a low detection limit at the picogram per microliter (pg/μL) level.
• Demonstrated a wide analytical range from 6.75 pg/μL to 67.5 ng/μL.
• The intensity ratio of specific Raman bands indicated mutation presence with high selectivity.

Conclusions:

• The developed DNA SERS biosensor enables rapid and precise identification of the BRAF V600E mutation.
• The nanostructured GaN-based platform offers high sensitivity, selectivity, and reproducibility.
• This bioactive platform shows potential for clinical sample analysis and personalized medicine.