Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

9.0K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
9.0K
Interference and Diffraction02:18

Interference and Diffraction

52.0K
Interference is a characteristic phenomenon exhibited by waves. When two electromagnetic waves interact with their peaks and troughs coinciding, a resulting wave with enhanced amplitude is produced. This is known as constructive interference. In this case, the two waves interacting are in phase with each other.
52.0K
Group Polarization01:01

Group Polarization

38.4K
Group polarization is the strengthening of an original group attitude following the discussion of views within a group (Teger & Pruitt, 1967). That is, if a group initially favors a viewpoint, after discussion the group consensus is likely a stronger endorsement of the viewpoint. Conversely, if the group was initially opposed to a viewpoint, group discussion would likely lead to stronger opposition.
38.4K
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

12.9K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
12.9K
RNA Interference01:23

RNA Interference

28.0K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
28.0K
Molecular Shape and Polarity03:37

Molecular Shape and Polarity

75.0K
Dipole Moment of a Molecule
75.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Isotropic shrinkage of patterned vacancies enables three-dimensional nanoprecise metastructures for visible light applications.

Nature photonics·2026
Same author

Workshop on Noninvasive Glucose Monitoring 2025.

Journal of diabetes science and technology·2026
Same author

Clinical Validation on Healthy Humans of a Portable Non-invasive Continuous Glucose Monitor Based on Transdermal Band-Pass Raman Spectroscopy.

Journal of diabetes science and technology·2026
Same author

Scanless temporal focusing enables high-speed three-dimensional quantitative phase microscopy.

Research square·2026
Same author

Interfacial polarity modulation of positive electrode active materials for high-potential lithium metal batteries.

Nature nanotechnology·2026
Same author

Aging changes cell mechanics and dynamics associated with cytoplasmic crowding.

PNAS nexus·2026
Same journal

Application of Resonant Plasmonic Bowtie Nanoantennas for Optically-Assisted Diffusiophoretic Trapping of Extracellular Vesicles and Nanoparticles.

Laser & photonics reviews·2026
Same journal

Ultranarrow linewidth photonic-atomic laser.

Laser & photonics reviews·2026
Same journal

Free-Space-Coupled Frequency-Locked Microtoroid Resonators with Reactive Polymer Functionalization for Part-Per-Trillion Gas Detection.

Laser & photonics reviews·2026
Same journal

On-Chip Multimode WGM Microresonator with Cross-Correlation Algorithm for Enhanced Sensing.

Laser & photonics reviews·2025
Same journal

Cavity Optomechanical Bistability with an Ultrahigh Reflectivity Photonic Crystal Membrane.

Laser & photonics reviews·2025
Same journal

Pixelation with concentration-encoded effective photons for quantitative molecular optical sectioning microscopy.

Laser & photonics reviews·2025
See all related articles

Related Experiment Video

Updated: Jan 27, 2026

Hyperspectral Imaging as a Tool to Study Optical Anisotropy in Lanthanide-Based Molecular Single Crystals
07:24

Hyperspectral Imaging as a Tool to Study Optical Anisotropy in Lanthanide-Based Molecular Single Crystals

Published on: April 14, 2020

18.5K

Single-Shot Optical Anisotropy Imaging with Quantitative Polarization Interference Microscopy.

Baoliang Ge1, Renjie Zhou2, Yu Takiguchi3

  • 1Department of Mechanical Engineering Massachusetts Institute of Technology Cambridge, MA 02139, USA, Department of Biological Engineering Massachusetts Institute of Technology Cambridge, MA 02139, USA, Laser Biomedical Research Center Massachusetts Institute of Technology Cambridge, MA 02139, USA.

Laser & Photonics Reviews
|March 23, 2019
PubMed
Summary
This summary is machine-generated.

Quantitative polarization interference microscopy (QPIM) enables single-shot mapping of birefringence parameters. This novel technique accurately measures retardance and orientation, ideal for dynamic material analysis.

Keywords:
instrumentationinterference microscopymeasurement and metrologymicroscopypolarimetric imaging

More Related Videos

Phase Contrast and Differential Interference Contrast DIC Microscopy
06:49

Phase Contrast and Differential Interference Contrast DIC Microscopy

Published on: August 6, 2008

54.0K
Time-Resolved Fluorescence Anisotropy from Single Molecules for Characterizing Local Flexibility in Biomolecules
10:23

Time-Resolved Fluorescence Anisotropy from Single Molecules for Characterizing Local Flexibility in Biomolecules

Published on: April 25, 2025

1.0K

Related Experiment Videos

Last Updated: Jan 27, 2026

Hyperspectral Imaging as a Tool to Study Optical Anisotropy in Lanthanide-Based Molecular Single Crystals
07:24

Hyperspectral Imaging as a Tool to Study Optical Anisotropy in Lanthanide-Based Molecular Single Crystals

Published on: April 14, 2020

18.5K
Phase Contrast and Differential Interference Contrast DIC Microscopy
06:49

Phase Contrast and Differential Interference Contrast DIC Microscopy

Published on: August 6, 2008

54.0K
Time-Resolved Fluorescence Anisotropy from Single Molecules for Characterizing Local Flexibility in Biomolecules
10:23

Time-Resolved Fluorescence Anisotropy from Single Molecules for Characterizing Local Flexibility in Biomolecules

Published on: April 25, 2025

1.0K

Area of Science:

  • Optics and Photonics
  • Materials Science
  • Biomedical Imaging

Background:

  • Optical anisotropy is crucial for characterizing materials and biological samples.
  • Existing methods for birefringence measurement can be time-consuming or lack single-shot capabilities.

Purpose of the Study:

  • To introduce a novel single-shot polarized light imaging technique for mapping birefringence parameters.
  • To develop a mathematical model for quantitative polarization interference microscopy (QPIM).

Main Methods:

  • Designed a compact polarization-resolved interference microscopy system for single-shot data acquisition.
  • Developed a mathematical model to extract retardance and orientation maps from interferograms.
  • Validated the system using a calibrated quarter-wave plate.

Main Results:

  • Achieved accurate determination of the fast-axis orientation angle and retardance of a quarter-wave plate.
  • Demonstrated the capability for single-shot measurement of transient birefringence dynamics.
  • Captured dynamic retardance changes in a nematic liquid crystal device.

Conclusions:

  • QPIM offers a powerful, single-shot approach for quantitative birefringence mapping.
  • The technique is suitable for analyzing static anisotropic properties and dynamic changes.
  • QPIM has potential applications in material science and biological imaging.