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Updated: Jan 27, 2026

A Burrowing/Tunneling Assay for Detection of Hypoxia in Drosophila melanogaster Larvae
Published on: March 27, 2018
Hypoxia Reporter Element Assay
Daelynn R Buelow1, Sharyn D Baker1
1Division of Pharmaceutics, College of Pharmacy and Comprehensive Cancer Center, The Ohio State University, Columbus, Ohio, USA.
Abstract:
Hypoxia is a condition in which there is a decrease in oxygen supply to the cellular environment. Changes to cellular oxygen levels can lead to transcriptional changes of oxygen-regulated genes. Reporter assays are used to study gene expression alteration and modifications in response to environmental changes. Dual-reporter assays allow the simultaneous measurement of two different genes within a single cell, thus improving experimental accuracy. Within this protocol, we describe the utilization of the LightSwitch Dual Assay System to measure BMX expression in response to hypoxic conditions.
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