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Updated: Jan 27, 2026

Deacetylation Assays to Unravel the Interplay between Sirtuins SIRT2 and Specific Protein-substrates
Published on: February 27, 2016
[Sirtuins and their role in metabolism regulation]
Zuzanna Frydzińska1, Aleksandra Owczarek1, Katarzyna Winiarska1
1Zakład Regulacji Metabolizmu, Instytut Biochemii, Wydział Biologii, Uniwersytet Warszawski, Warszawa, Polska.
Abstract:
Sirtuins are - in mammals - a family of seven enzymes (sirtuin 1-7) involved in post-translational modification of proteins (mainly deacetylation, but also: polyADP-ribosylation, demalonylation or lipoamidation), and thus - in the regulation of many metabolic processes. The activity of all sirtuins depends on the availability of NAD+. However, the function of individual isoforms is different, even mutually antagonistic. In this article the role of sirtuins in the regulation of glucose and lipid metabolism and in DNA repair mechanisms is described in detail. The significance of these enzymes in diseases pathogenesis, with particular emphasis on diabetes and cancer, is also discussed, indicating the possible therapeutic use of sirtuin activity modulators.
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