Cu²⁺-assisted laccase from Trametes versicolor enhanced self-polyreaction of triclosan

Laccase-mediated humification processes (L-MHPs) can be used to polymerize and transform phenolic pollutants in water. However, the mechanism on Cu²⁺ impacts the self-polymerization of multi-purpose antimicrobial agent triclosan during L-MHPs is less understood. Here, the influence of divalent metal ions (DMIs) on Trametes versicolor laccase activity was investigated. Particularly, the performance of Cu²⁺-assisted laccase in polymerizing and transforming triclosan was systematically characterized. Compared with DMI-free, the activity of laccase was obviously accelerated with Cu²⁺ present due to copper is a vital component of laccase catalytic center. It was found that Cu²⁺-assisted laccase was effective in transforming triclosan, and the enzymatic reaction kinetic constants increased from 0.28 to 0.73 h^-1 as the Cu²⁺ concentration increased (0-3.0 mM). Identification of intermediate products revealed that laccase oxidation predominantly generated triclosan dimers, trimers, and tetramers. The presence of Cu²⁺ reinforced self-polymerization of triclosan via forming more triclosan oligomers relative to the Cu²⁺-free, which likely attributed to the enhancement of laccase activity and stability with Cu²⁺ present in L-MHPs. A possible transformation mechanism was proposed as follows: Laccase initially catalyzed the oxidation of triclosan to generate phenoxy radical intermediates, which self-coupled to each other subsequently by radical-mediated CC and COC covalent binding, forming oligomers and polymers. The growth inhibitory assays of freshwater microalgae (Chlamydomonas reinhardtii and Scenedesmus obliquus) demonstrated that the self-polymerized triclosan by L-MHPs had lower toxicity than the parent compound. These findings implied that Cu²⁺-assisted laccase was an effective strategy for rapidly self-polyreaction and detoxication of triclosan from Cu²⁺-triclosan combined polluted wastewater.