Related Experiment Video
Updated: Aug 18, 2026

Culturing Primary Rat Inner Medullary Collecting Duct Cells
Published on: June 21, 2013
Angiotensin II stimulates inositol phosphate formation in rat anterior pituitary glands
Abstract:
The effect of angiotensin II (AII) on polyphosphoinositide (PPI) turnover at the pituitary was studied. When [3H]inositol-prelabelled rat anterior hemipituitaries were incubated with AII (10 microM) for 30 min in the presence of Li+ (10 mM), the production of IP, IP2 and IP3 were increased to 182, 199 and 158% of paired control values. Time course studies showed that the stimulation of IP3 formation by AII was significant after 2 min incubation and preceded the accumulation of the other inositol phosphates. The specificity of the AII effect was confirmed by inhibition with the specific AII antagonist, saralasin. These findings suggest that AII action at the anterior pituitary can be mediated via the early hydrolysis of PPI.
Related Concept Videos
Endocrine Signaling
Hormonal Regulation
Phosphoinositides and PIPs
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
IP3/DAG Signaling Pathway
Nitric Oxide Signaling Pathway
Transducer Mechanism: Enzyme-Linked Receptors
Major types that are helpful drug targets include:

