Related Experiment Video
Updated: Jan 27, 2026
11:58
A Protocol for Roux-en-Y Gastric Bypass in Rats using Linear Staplers
Published on: August 21, 2021
5.9K
Roux-en-Y gastric bypass surgery reprograms enterocyte triglyceride metabolism and postprandial secretion in rats
Sharon Kaufman1, Myrtha Arnold1, Abdiel Alvarado Diaz2
1Physiology and Behavior Laboratory, ETH Zurich, Schwerzenbach, Switzerland.
Molecular Metabolism
|March 26, 2019
Summary
Roux-en-Y gastric bypass (RYGB) surgery significantly reduces intestinal triglyceride (TG) secretion by altering how enterocytes handle lipids. This mechanism contributes to lower plasma TG levels and improved cardiovascular health in patients.
Area of Science:
- Metabolic Surgery
- Gastroenterology
- Lipid Metabolism
Background:
- Roux-en-Y gastric bypass (RYGB) effectively lowers plasma triglyceride (TG) levels, reducing cardiovascular risk.
- The precise mechanisms behind RYGB's impact on systemic TG reduction are not fully understood.
Purpose of the Study:
- To investigate if RYGB surgery alters intestinal triglyceride secretion through changes in enterocyte lipid handling.
- To elucidate the molecular and functional modifications in enterocytes post-RYGB.
Main Methods:
- Diet-induced obese rats underwent RYGB or Sham surgery.
- Intestinal lymph was analyzed for TG levels and lipid profiles using mass spectrometry.
- Enterocyte lipid handling, including absorption, reesterification, and storage, was assessed via transcriptional and functional assays.
Main Results:
- RYGB significantly reduced postprandial TG concentrations in both plasma and intestinal lymph compared to Sham surgery.
- Lymphatic TG reduction was more pronounced than other lipid subclasses, with a shift towards unsaturated TG species.
- Enterocytes in RYGB rats showed reduced lipid uptake and upregulated TG reesterification genes.
Conclusions:
- RYGB surgery substantially decreases intestinal TG secretion and modifies enterocyte lipid metabolism in rats.
- These enterocyte-level changes are likely key contributors to the improved plasma TG profiles observed after RYGB in humans.
Related Concept Videos
00:52What is Metabolism?
131.5K
Overview
131.5K
01:16Gastric Motility
3.0K
Gastric motility is the coordinated contraction and relaxation of stomach muscles that convert ingested food into chyme, a semi-liquid substance ready for further digestion in the intestines. The process begins with the vagus nerve inducing the relaxation of the smooth muscles in the fundus and body of the stomach, allowing these regions to expand and accommodate up to approximately 1.5 liters of food and liquid.
Peristaltic Waves and Chyme Formation
Upon food entry, the stomach initiates...
Peristaltic Waves and Chyme Formation
Upon food entry, the stomach initiates...
3.0K
01:16Gastric Emptying
3.6K
Gastric emptying occurs when the stomach gradually releases chyme into the duodenum. When the stomach is distended, it triggers the release of gastrin, a hormone that promotes gastric acid secretion to aid in digestion. Additionally, stomach distension contributes to peristaltic waves that propel gastric contents toward the pyloric region. The gastroenteric reflex, on the other hand, primarily stimulates peristalsis in the intestines, facilitating the movement of contents further along the...
3.6K
01:26Gastric Phase of Digestion
3.2K
The gastric phase of digestion begins as soon as food enters the stomach. The incoming food bolus triggers neural and hormonal mechanisms, which last approximately 3 to 4 hours. During this phase, the stomach undergoes significant changes to prepare the food for further digestion and absorption.
When food enters the stomach, it stretches the stomach walls and activates stretch receptors. This triggers local reflexes of the enteric nervous system, mediated through the myenteric plexus. These...
When food enters the stomach, it stretches the stomach walls and activates stretch receptors. This triggers local reflexes of the enteric nervous system, mediated through the myenteric plexus. These...
3.2K
01:37Neural Regulation
43.3K
Digestion begins with a cephalic phase that prepares the digestive system to receive food. When our brain processes visual or olfactory information about food, it triggers impulses in the cranial nerves innervating the salivary glands and stomach to prepare for food.
43.3K
01:14Introduction to Nuclear Reprogramming
2.3K
Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...
2.3K