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Updated: Jan 27, 2026

Mammalian Cell Encapsulation in Alginate Beads Using a Simple Stirred Vessel
Published on: June 29, 2017
Tridimensional alginate disks of tunable topologies for mammalian cell encapsulation
Alejandra Suarez-Arnedo1, Diana M Narváez1, Paula Sarmiento2
1Human Genetics Laboratory, Department of Biological Sciences, Universidad de los Andes, Bogotá, Colombia.
Abstract:
We have developed a protocol to produce three-dimensional matrices based on alginate hydrogels for mammalian cell encapsulation. Based on the gelation properties of this polysaccharide, we implemented a calcium ion-based diffusion method where the designed hydrogels can be obtained with well-defined mechanical properties and replicable 3D topologies. The developed protocol can be extended to different types of alginates and an ample range of concentrations. This makes it very attractive for various biomedical applications where strict control over structure-function relationships is desirable.
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