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Updated: Jan 27, 2026

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Fibroblast Growth Factor 1 Promotes Rat Stem Leydig Cell Development.

Lanlan Chen1,2, Xiaoheng Li1, Yiyan Wang1

  • 1Department of Anesthesiology, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, China.

Frontiers in Endocrinology
|March 26, 2019
PubMed
Summary

Fibroblast growth factor 1 (FGF1) promotes stem Leydig cell proliferation in rats. However, FGF1 also inhibits the differentiation of these stem cells into mature Leydig cells.

Keywords:
differentiationfibroblast growth factor 1proliferationstem leydig celltestosterone

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Area of Science:

  • Reproductive Biology
  • Endocrinology
  • Cell Biology

Background:

  • Fibroblast growth factor 1 (FGF1) expression is noted in the testis.
  • The specific role of FGF1 in stem Leydig cell development is not well understood.

Purpose of the Study:

  • To investigate the effects of FGF1 on rat stem Leydig cell development using an ethane dimethane sulfonate (EDS)-induced injury model.

Main Methods:

  • Intratesticular injection of FGF1 in an EDS-treated rat model.
  • Assessment of serum testosterone levels, Leydig cell proliferation (PCNA-positive cells), and Leydig cell number.
  • Analysis of gene expression for key Leydig cell markers.
  • Primary culture of seminiferous tubules to evaluate FGF1's effect on stem Leydig cell proliferation and differentiation (EdU incorporation).

Main Results:

  • FGF1 administration increased serum testosterone, Leydig cell proliferation, and Leydig cell number.
  • FGF1 down-regulated the expression of crucial Leydig cell steroidogenic enzymes and receptors (Lhcgr, Star, Cyp11a1, Hsd3b1, Cyp17a1, Hsd11b1).
  • In vitro, FGF1 stimulated stem Leydig cell proliferation but inhibited their differentiation, potentially via FGFR1 signaling.

Conclusions:

  • FGF1 acts as a mitogen for stem Leydig cells, enhancing their proliferation.
  • FGF1 impedes the differentiation of stem Leydig cells into mature Leydig cells, suggesting a regulatory role in maintaining the stem cell pool.