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Related Concept Videos

Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

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Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
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Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

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Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...
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Mass Spectrometry of Amines01:15

Mass Spectrometry of Amines

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In mass spectroscopy, amines undergo fragmentation to give parent ions with odd molecule weights. This observed mass spectrum follows the nitrogen rule; a molecule with an odd number of nitrogen atoms produces a molecular ion with an odd molecular weight. Amines undergo fragmentation through α cleavage, producing nitrogen-containing cations—iminium ions—and alkyl radicals. Mass spectra of aromatic and cyclic aliphatic amines exhibit strong molecular ion peaks, but acyclic...
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Mass Spectrometry: Isotope Effect01:13

Mass Spectrometry: Isotope Effect

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Most elements exist in nature as a mixture of isotopes. The isotopes differ in weight due to their respective number of neutrons. The molecular weight of a molecule is different depending on the specific isotope of its elements involved. As a result, the mass spectrum of the molecule exhibits peaks from the same fragment at multiple positions. The positions of these mass signals depend on the mass differences between isotopes. Furthermore, the intensity of these signals is dependent on the...
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MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

6.6K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
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Chemical Ionization (CI) Mass Spectrometry01:21

Chemical Ionization (CI) Mass Spectrometry

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The molecular ion peak of a molecule in the mass spectrum provides vital information for molecular identification. However, conventional electron impact ionization can lead to the rapid dissociation of some molecular ions before they reach the detector. A milder ionization method is required to increase the lifetime of such ionized analyte molecules. Chemical ionization (CI) is a gas-phase protonation reaction useful for mass-analyzing analyte molecules that are easily protonated to yield the...
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Updated: Jan 27, 2026

Semi-Quantitative Analysis of Peptidoglycan by Liquid Chromatography Mass Spectrometry and Bioinformatics
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Mass spectrometry-based intraoperative tumor diagnostics.

Lorena Hänel1,2,1,2, Marcel Kwiatkowski1,3,1,3, Laura Heikaus1,1

  • 1Institute of Clinical Chemistry and Laboratory Medicine, University Medical Center Hamburg-Eppendorf, Martinistr. 52, 20246 Hamburg, Germany.

Future Science OA
|March 26, 2019
PubMed
Summary

Mass spectrometry innovations are improving intraoperative tumor margin localization in surgical oncology. Real-time diagnosis using technologies like the intelligent knife and MasSpec pen is becoming achievable for better surgical decisions.

Keywords:
DESIMasSpec penPIRLcanceriKnifeintraoperative tumor diagnosticsreal-time diagnosissurgerytumor margin

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Area of Science:

  • Surgical Oncology
  • Analytical Chemistry
  • Medical Technology

Background:

  • Accurate tumor margin assessment is critical in surgical oncology to balance tissue preservation with complete tumor removal.
  • Intraoperative decisions directly impact patient outcomes and the need for further treatments.

Purpose of the Study:

  • To review recent technical advancements in mass spectrometry for precise tumor margin localization.
  • To critically evaluate the current status of these technologies in scientific and clinical practice.

Main Methods:

  • Review of mass spectrometry-based innovations for intraoperative tumor margin detection.
  • Focus on specific technologies: intelligent knife, desorption electrospray ionization (DESI), picosecond infrared laser (PIRL), and MasSpec pen.

Main Results:

  • Mass spectrometry offers promising real-time diagnostic capabilities for surgical margins.
  • Several innovative techniques show potential for enhanced accuracy in differentiating tumor from healthy tissue.

Conclusions:

  • Emerging mass spectrometry technologies are nearing clinical application for real-time intraoperative tumor margin analysis.
  • Further evaluation is needed to integrate these advancements into standard surgical practice.