Multiplex protein detection on circulating tumor cells from liquid biopsies using imaging mass cytometry

Area of Science:

• Oncology
• Biotechnology
• Medical Diagnostics

Background:

• Molecular analysis of circulating and disseminated tumor cells (CTCs/DTCs) offers potential for real-time monitoring of cancer prognosis and treatment efficacy via liquid biopsies.
• Current methods require further development and validation for robust molecular analysis of these rare cells.

Purpose of the Study:

• To integrate imaging mass cytometry (IMC) into the High Definition Single Cell Analysis (HD-SCA) assay for liquid biopsies.
• To develop and validate methods for image analysis and signal normalization for IMC-based liquid biopsies.

Main Methods:

• Integration of Fluidigm Hyperion Imaging System's IMC with metal-labeled antibodies into the HD-SCA workflow.
• Application to liquid biopsies from a metastatic prostate cancer patient.
• Development of image analysis and signal normalization techniques.

Main Results:

• Demonstrated successful integration of IMC for extended CTC characterization.
• Enabled analysis of dozens of protein biomarkers on CTCs.
• Showcased potential for understanding biological properties influencing therapeutic response, metastasis, and immune surveillance.

Conclusions:

• IMC significantly enhances the molecular profiling capabilities of liquid biopsies for CTCs.
• This integrated approach provides deeper insights into cancer biology and patient outcomes.
• Further development holds promise for improved cancer management strategies.