Challenges in measurement of linkage following HIV self-testing: examples from the STAR Project

Overview
Phosphodiester bond forms when a phosphoric acid molecule (H3PO4) links with two hydroxyl groups (–OH) of two other molecules, forming two ester bonds. Two water molecules are released in this process. The phosphodiester bond is commonly found in nucleic acids (DNA and RNA) and plays a critical role in their structure and function.
Phosphodiester Bonds Link Nucleotides Together
DNA and RNA are polynucleotides or long chains of nucleotides that are linked together. A nucleotide is...

Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked.  In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence...

Newton's third law states that every action has an equal and opposite reaction. Consider a swimmer pushing off the side of a pool. They push against the wall of the pool with their feet and accelerate in the direction opposite to that of their push. This occurs because the wall exerts an equal and opposite force on the swimmer. Here, the forces do not cancel out each other as they are acting on different systems. In this case, there are two systems: the swimmer and the wall. If we select...

Solving problems that involve forces is easy using free-body diagrams. A free-body diagram is a sketch showing all the external forces that are acting on an object or system. The object or system is represented by a single isolated point (or free body). Only those forces acting on it that originate outside of the object or system—the external forces—are shown. The forces are represented by vectors extending outward from the free body. Imagine a person sitting on a chair. Here, the...

Learning to draw Fischer projections of molecules and understanding their relevance plays a crucial role in the visual depiction of organic molecules. A Fischer projection is a two-dimensional projection on a planar surface to simplify the three-dimensional wedge–dash representation of molecules. This is especially helpful in the case of molecules with multiple chiral centers that can be difficult to draw. Here, all the bonds of interest are represented as horizontal or vertical lines. While...