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[Decontamination of continual cell lines spontaneously infected with mycoplasmas]
Abstract:
The continual cell lines of bovine kidneys MDBK and AUBEK, and porcine kidneys RPD and IBRS, spontaneously infected with Mycoplasma arginini and Acholeplasma laidlawii, were decontaminated by the method of selective elimination. Two elimination procedures were modified to be used for the decontamination: one based on the reduction of infection by the light treatment of the cultures, the other based on the selection of mycoplasma-free cell population through cell clonation. On the basis of a long-continued control of the cell clones a methodical procedure of the preparation of mycoplasma-free cell lines was worked out.
Insights
This study developed a selective elimination method to remove Mycoplasma contamination from bovine and porcine kidney cell lines. The successful decontamination process ensures the preparation of mycoplasma-free cell lines for research.
Area of Science:
- Veterinary Microbiology
- Cell Biology
- Biotechnology
Context:
- Spontaneous contamination of continuous cell lines with Mycoplasma arginini and Acholeplasma laidlawii is a common issue in cell culture.
- Bovine kidney cell lines (MDBK, AUBEK) and porcine kidney cell lines (RPD, IBRS) were identified as infected.
- Mycoplasma contamination can significantly affect cell line characteristics and experimental results.
Purpose:
- To develop and validate effective decontamination methods for mycoplasma-infected cell lines.
- To establish a reliable procedure for preparing mycoplasma-free cell lines.
- To provide a solution for researchers relying on contaminated cell lines.
Summary:
- Two selective elimination procedures were modified: light treatment of cultures and cell cloning for mycoplasma-free population selection.
- Continuous monitoring of cell clones was performed to ensure successful decontamination.
- A methodical procedure for preparing mycoplasma-free cell lines was established based on long-term controls.
Impact:
- Enables the production of reliable and reproducible cell-based research by eliminating mycoplasma interference.
- Provides a validated protocol for decontaminating commonly used bovine and porcine kidney cell lines.
- Contributes to maintaining the integrity and quality of cell line repositories.