Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Properties of Enantiomers and Optical Activity02:24

Properties of Enantiomers and Optical Activity

21.5K
It is essential to understand the difference between chiral and achiral interactions and the implications thereof in optical activity and their applications. Just as our feet, which are chiral, interact uniquely with chiral objects, such as a pair of shoes, but identically with achiral socks, enantiomers of a molecule exhibit different properties only when they interact with other chiral media. An example of a significant implication from this facet is the phenomenon known as optical activity,...
21.5K
Control System Problem01:21

Control System Problem

422
In an open-loop system, such as a basic thermostat, the poles of the transfer function influence the system's response but do not determine its stability. However, when feedback is introduced to form a closed-loop system, such as an advanced thermostat that adjusts heating based on room temperature, stability is governed by the new poles of the closed-loop transfer function.
When forming a closed-loop system, issues can arise if the poles cross into the unstable region, leading to potential...
422
Types of Biopharmaceutical Studies: Controlled and Non-Controlled Approaches01:23

Types of Biopharmaceutical Studies: Controlled and Non-Controlled Approaches

420
Biopharmaceutical studies constitute a vital field aiming to enhance drug delivery methods and refine therapeutic approaches, drawing upon diverse interdisciplinary knowledge. In research methodologies, the choice between controlled and non-controlled studies significantly influences the study's reliability and accuracy.
Non-controlled studies, commonly employed for initial exploration, lack a control group, rendering them susceptible to biases and external influences. In contrast,...
420
Combinatorial Gene Control02:33

Combinatorial Gene Control

9.5K
Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
9.5K
Control Systems01:10

Control Systems

1.8K
Control systems are everywhere in contemporary society, influencing diverse applications from aerospace to automated manufacturing. These systems can be found naturally within biological processes, such as blood sugar regulation and heart rate adjustment in response to stress, as well as in man-made systems like elevators and automated vehicles. A control system is essentially a network of subsystems and processes that collaboratively convert specific inputs into desired outputs.
At the heart...
1.8K
Controller Configurations01:22

Controller Configurations

367
Controller configurations are crucial in a car's cruise control system because they manage speed over time to maintain a consistent pace regardless of road conditions, thereby meeting design goals. In traditional control systems, fixed-configuration design involves predetermined controller placement. System performance modifications are known as compensation.
Control-system compensation involves various configurations, most commonly series or cascade compensation, in which the controller...
367

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Clinician Role in Decarbonizing Outpatient and Inpatient Health Care Settings.

Pediatric clinics of North America·2026
Same author

Rapid colorimetric antimicrobial susceptibilities direct from positive blood culture for Gram-negative bacteria.

Microbiology spectrum·2025
Same author

Are Children's Hospitals Doing Enough to Address the Climate Crisis?

Hospital pediatrics·2024
Same author

Sequential Two-Photon Delayed Fluorescence Anisotropy for Macromolecular Size Determination.

The journal of physical chemistry. B·2023
Same author

Rapid, label-free antibiotic susceptibility determined directly from positive blood culture.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2022
Same author

Timing Is Everything.

Pediatric critical care medicine : a journal of the Society of Critical Care Medicine and the World Federation of Pediatric Intensive and Critical Care Societies·2022

Related Experiment Video

Updated: Jan 27, 2026

Determining if DNA Stained with a Cyanine Dye Can Be Digested with Restriction Enzymes
06:58

Determining if DNA Stained with a Cyanine Dye Can Be Digested with Restriction Enzymes

Published on: February 2, 2018

9.2K

Optically Activated Delayed Fluorescence through Control of Cyanine Dye Photophysics.

Daniel P Mahoney1, Aida A Demissie1, Robert M Dickson1

  • 1School of Chemistry & Biochemistry and Petit Institute of Bioengineering and Bioscience , Georgia Institute of Technology , Atlanta , Georgia 30332 , United States.

The Journal of Physical Chemistry. A
|March 26, 2019
PubMed
Summary

Researchers developed optically activated delayed fluorescence (OADF) in organic dyes. This novel technique enhances fluorescence imaging by using a secondary laser to repopulate the emissive state from a dark triplet state.

More Related Videos

Optical Recording of Electrical Activity in Guinea-pig Enteric Networks using Voltage-sensitive Dyes
14:23

Optical Recording of Electrical Activity in Guinea-pig Enteric Networks using Voltage-sensitive Dyes

Published on: December 4, 2009

10.3K
Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
07:44

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes

Published on: July 6, 2016

11.6K

Related Experiment Videos

Last Updated: Jan 27, 2026

Determining if DNA Stained with a Cyanine Dye Can Be Digested with Restriction Enzymes
06:58

Determining if DNA Stained with a Cyanine Dye Can Be Digested with Restriction Enzymes

Published on: February 2, 2018

9.2K
Optical Recording of Electrical Activity in Guinea-pig Enteric Networks using Voltage-sensitive Dyes
14:23

Optical Recording of Electrical Activity in Guinea-pig Enteric Networks using Voltage-sensitive Dyes

Published on: December 4, 2009

10.3K
Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
07:44

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes

Published on: July 6, 2016

11.6K

Area of Science:

  • Photophysics
  • Organic Dyes
  • Fluorescence Spectroscopy

Background:

  • Merocyanine 540 fluorescence is typically enhanced by optically depopulating dark photoisomer states.
  • Existing methods focus on regenerating the fluorescence-generating manifold of the all-trans isomer.

Purpose of the Study:

  • To explore a competing modulation route for enhancing fluorescence.
  • To generate optically activated delayed fluorescence (OADF) using long-wavelength coexcitation.
  • To investigate the potential for new high-sensitivity fluorescence imaging schemes.

Main Methods:

  • Utilized long-wavelength (637 nm) continuous wave coexcitation to depopulate the trans triplet population.
  • Observed OADF (∼580 nm) with pulsed fluorescence excitation at 532 nm.
  • Investigated the effect of secondary laser intensity on OADF decay rates.

Main Results:

  • Successfully generated OADF from organic dyes for the first time.
  • Demonstrated that OADF is a result of reverse intersystem crossing (RISC) from a triplet state to the emissive S1 state.
  • Observed ns-lived fluorescence delayed by microseconds after primary excitation.
  • Showed that OADF decay rate increases with secondary laser intensity.

Conclusions:

  • Optically activated delayed fluorescence (OADF) can be generated in organic dyes.
  • This method offers background-free collection and potential for high-sensitivity fluorescence imaging.
  • The observed OADF mechanism involves sequential two-photon excitation and repumped fluorescence.