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Quantitative Analysis of Cellular Composition in Advanced Atherosclerotic Lesions of Smooth Muscle Cell Lineage-Tracing Mice
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ImmunoSERS microscopy for the detection of smooth muscle cells in atherosclerotic plaques

Ewelina Wiercigroch1, Elzbieta Stepula2, Lukasz Mateuszuk3

  • 1Faculty of Chemistry, Jagiellonian University, Gronostajowa 2, Krakow 30-387, Poland; Jagiellonian Centre for Experimental Therapeutics (JCET), Jagiellonian University, Bobrzynskiego 14, Krakow 30-348, Poland.

Biosensors & Bioelectronics
|March 26, 2019
PubMed

We investigated the suitability of immuno-SERS (iSERS) microscopy for imaging of smooth muscle cells (SMCs) in atherosclerotic plaques. Localization of SMCs is achieved by using SERS-labelled antibodies direct against alpha-smooth muscle actin (SMA). The staining quality of the false-colour iSERS images obtained by confocal Raman microscopy with point mapping is compared with wide-field immunofluorescence images. Both direct (labelled primary antibody) and indirect iSERS staining (unlabelled primary and labelled secondary antibody) techniques were employed. Direct iSERS staining yields results comparable to indirect IF staining, demonstrating the suitability of iSERS in research on atherosclerosis and paving the way for future multiplexed imaging experiments.

Keywords:
AtherosclerosisDirect/indirect stainingImmunoSERS microscopySmooth muscle cells (SMCs)

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