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Cobalamin is produced by Acetobacter pasteurianus DSM 3509
Clemens Bernhardt1, Xuan Zhu1,2, David Schütz3
1Hamburg School of Food Science, Biocenter Klein Flottbek, Division of Food Microbiology and Biotechnology, University of Hamburg, Ohnhorststr. 18, 22609, Hamburg, Federal Republic of Germany.
Applied Microbiology and Biotechnology
|March 27, 2019
Summary
Acetobacter pasteurianus DSM 3509 can produce cobalamin (vitamin B12), a crucial nutrient. This finding suggests potential cobalamin in foods fermented with this bacterium.
Area of Science:
- Microbiology
- Food Science
- Biochemistry
Background:
- Limited cobalamin-producing bacteria are suitable for food fermentation.
- Acetobacter pasteurianus DSM 3509's potential for cobalamin synthesis was investigated.
Purpose of the Study:
- To confirm cobalamin synthesis by Acetobacter pasteurianus DSM 3509.
- To characterize the synthesized cobalamin and assess its presence in fermented foods.
Main Methods:
- Microbiological assay using Lactobacillus delbrueckii ssp. lactis DSM 20355.
- Immunoaffinity chromatography for product isolation.
- Mass spectrometry for fragmentation pattern analysis.
Main Results:
- Acetobacter pasteurianus DSM 3509 synthesized cyanocorrinoids and noncorrinoid growth factors.
- Isolated product matched cyanocobalamin's UV spectrum and mass spectrometry fragmentation.
- Synthesized cobalamin differed from pseudovitamin B12.
Conclusions:
- Acetobacter pasteurianus DSM 3509 is a cobalamin-producing bacterium.
- Fermented foods using this strain may contain cobalamin residues.
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