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Benign odontogenic ghost cell lesions revisited and new considerations on dysplastic dentin
Ana Cláudia Garcia Rosa1,2, Lucas Novaes Teixeira3, Fabricio Passador-Santos3
1School of Medicine, Federal University of Tocantins, Quadra 109 Norte, Avenida NS-15, ALCNO-14, Plano Diretor Norte, Palmas, Tocantins, 77001-090, Brazil. anaclaudiagarcia@uft.edu.br.
Objectives:
This study aimed to revisit benign odontogenic ghost cell lesions (BOGCL) by hematoxylin and eosin staining and immunohistochemistry.
Materials And Methods:
Thirty cases of calcifying odontogenic cyst (COC) and 6 cases of dentinogenic ghost cell tumor (DGCT) were selected for histopathological and immunohistochemical analysis. Sections stained for cytokeratin (K) 14, K-19, amelogenin, collagen type 1 (COL-1), and dentin matrix acidic phosphoprotein 1 (DMP-1) were evaluated using qualitative analysis. Sections stained for Ki-67 and minichromosome maintenance protein-2 (MCM-2) were evaluated using semi-quantitative analysis.
Results:
A morphologic overlap was noticed in all BOGCL. Moreover, no differences were detected in the expression of K-14 and K-19. The expression of proliferative markers Ki-67 and MCM-2 was similar between cystic and tumor lesions (p > .05). The presence of COL-1 and absence of amelogenin in the so-called dysplastic dentin, associated with its histologic pattern, suggest that this is in fact an enameloid-like tissue.
Conclusions:
The dysplastic dentin should be considered an enameloid-like tissue in these lesions.
Clinical Relevance:
The similarity in histology, protein expression, and proliferative marker indices between COC and DGCT suggest that they are a sole entity and likely represent types of the same neoplasia.
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