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Updated: Jan 27, 2026

Retroviral Transduction of T-cell Receptors in Mouse T-cells
Published on: October 22, 2010
Assessment of Gene Function of Mouse Innate Lymphoid Cells for In Vivo Analysis Using Retroviral Transduction
Cyril Seillet1,2, Gabrielle T Belz3,4
1Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia. seillet@wehi.edu.au.
Abstract:
Retroviral transduction is commonly used to modulate gene expression and is a powerful approach to understand the role of a gene using gain- or loss-of-function strategies. Retroviral vectors can stably integrate non-viral genes into host genomes, providing long-term modulation of gene expression in infected cells and their progeny. Here we describe the generation of retroviral supernatants and the steps to efficiently transduce genes in innate lymphoid cell (ILC) progenitors for subsequent analysis of ILC populations in vivo.
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