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Related Concept Videos

CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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CRISPR/Cas9 Genome Editing01:28

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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In psychology, concepts can be divided into two categories: natural and artificial. Natural concepts are formed through direct or indirect experiences. For example, consider the concept of snow. If you live in a place with regular snowfall, such as Essex Junction, Vermont, you know snow through direct experiences. You’ve seen it fall, touched it, shoveled it, and played in it. You recognize its texture, appearance, and even its smell. In contrast, if you live on an island like Saint...
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The Antiviral System of Bacteria and Archaea: CRISPR01:23

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CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
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Related Experiment Video

Updated: Jan 27, 2026

Virus Delivery of CRISPR Guides to the Murine Prostate for Gene Alteration
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CRISPR-Cas9 Delivery by Artificial Virus (RRPHC).

Suleixin Yang1, Qinjie Wu1, Yuquan Wei1

  • 1State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu, China.

Methods in Molecular Biology (Clifton, N.J.)
|March 27, 2019
PubMed
Summary

CRISPR-Cas9 gene editing faces delivery challenges. Fabricating CRISPR-Cas9 plasmids into nanoparticles improves delivery efficiency for enhanced gene editing applications.

Keywords:
Artificial virusBranched polyethylene imineCRISPR-Cas9Heptafluorobutyric anhydrideTransfection

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Area of Science:

  • Molecular Biology
  • Biotechnology
  • Genetic Engineering

Background:

  • The CRISPR-Cas9 system has revolutionized gene editing since its inception.
  • Efficient delivery of CRISPR-Cas9 components (Cas9 protein and sgRNA) remains a significant technical hurdle.
  • Current DNA plasmid delivery methods for CRISPR-Cas9 are hampered by large size and low transfection efficiency.

Purpose of the Study:

  • To address the limitations of CRISPR-Cas9 plasmid delivery.
  • To develop an improved method for delivering CRISPR-Cas9 components into target cells.
  • To enhance gene editing efficiency through optimized delivery.

Main Methods:

  • Fabrication of CRISPR-Cas9 plasmids into a nanoparticle system.
  • Delivery of the nanoparticle-encapsulated CRISPR-Cas9 plasmids into target cells.
  • Evaluation of gene editing efficiency post-delivery.

Main Results:

  • Successful fabrication of CRISPR-Cas9 plasmids into nanoparticles.
  • Demonstrated efficient delivery of CRISPR-Cas9 components via the nanoparticle system.
  • Achieved effective gene editing in target cells.

Conclusions:

  • Nanoparticle-mediated delivery offers a promising solution to CRISPR-Cas9 delivery challenges.
  • This method overcomes the size and transfection efficiency limitations of conventional plasmid delivery.
  • The developed nanoparticle system enhances the broad applicability of CRISPR-Cas9 gene editing technology.