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Related Concept Videos

CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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Combinatorial Gene Control02:33

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Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
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Proteins: From Genes to Degradation02:11

Proteins: From Genes to Degradation

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Within a biological system, the DNA encodes the RNA, and the nucleotide sequence in the RNA further defines the amino acid sequence in the protein. This is referred to as “The Central Dogma of Molecular Biology” - a term coined by Francis Crick.  Central dogma is a firm principle in biology that defines the flow of genetic information within any life form. The two fundamental steps in central dogma are - transcription and translation.
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Gene Evolution - Fast or Slow?02:05

Gene Evolution - Fast or Slow?

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The genomes of eukaryotes are punctuated by long stretches of sequence which do not code for proteins or RNAs. Although some of these regions do contain crucial regulatory sequences, the vast majority of this DNA serves no known function. Typically, these regions of the genome are the ones in which the fastest change, in evolutionary terms, is observed, because there is typically little to no selection pressure acting on these regions to preserve their sequences.
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Updated: Jan 27, 2026

Highly Efficient Gene Disruption of Murine and Human Hematopoietic Progenitor Cells by CRISPR/Cas9
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Rapid and Efficient Gene Deletion by CRISPR/Cas9.

Signe Neldeborg1, Lin Lin2, Magnus Stougaard1

  • 1Department of Clinical Medicine, Aarhus University, Aarhus, Denmark.

Methods in Molecular Biology (Clifton, N.J.)
|March 27, 2019
PubMed
Summary

We present a rapid CRISPR-Cas9 gene editing method to generate knockout or deletion cells in under one month. This efficient technique streamlines DNA editing for various research applications.

Keywords:
DNADeletionEndonucleaseGene editingGenomic engineeringTransfectiongRNA

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Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9
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Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • CRISPR/Cas9 is a precise genetic engineering tool utilizing guide RNA (gRNA) for targeted DNA modifications.
  • It offers advantages over ZFNs and TALENs due to easily designed gRNAs for specific genomic loci.
  • CRISPR-Cas9 is widely adopted for gene editing applications like knockout, knockin, and tagging.

Purpose of the Study:

  • To describe a method for rapid and efficient generation of gene knockout or deletion cells using CRISPR/Cas9.
  • To achieve gene editing within a one-month timeframe.

Main Methods:

  • CRISPR/Cas9 system implementation for targeted genomic alterations.
  • Design of guide RNAs (gRNAs) for specific gene targeting.
  • Plasmid cloning, cell transfection, and culturing.
  • Selection and screening of positive clones for gene modification.

Main Results:

  • Successful generation of gene knockout or deletion cell lines.
  • Demonstration of CRISPR/Cas9 efficiency and speed within a one-month period.
  • Comprehensive methodology covering gRNA design to clone screening.

Conclusions:

  • The described method enables rapid and efficient CRISPR-Cas9 mediated gene editing.
  • This approach facilitates the swift generation of genetically modified cell lines for research.
  • The protocol offers a streamlined workflow for gene knockout and deletion studies.