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Fluorescence Enhancement from Nitro-Compound-Sensitive Bacteria within Spherical Hydrogel Scaffolds
Soohyun Kim, Hyunji Kim, Tian Qiao
1Department of Chemistry , Sejong University , 209, Neungdong-ro , Gwangjin-gu, Seoul 05006 , Republic of Korea.
ACS Applied Materials & Interfaces
|March 27, 2019
Summary
This study developed a bacterial sensor using hydrogel microbeads to detect explosive nitro compounds remotely. The enhanced hydrogel platform significantly amplified fluorescent signals for easier and more efficient explosive detection.
Area of Science:
- Biotechnology
- Materials Science
- Chemical Engineering
Background:
- Remote detection of explosive nitro compounds is crucial for safety.
- Bacterial sensors offer a sensitive detection method.
- Hydrogel microbeads can serve as a platform for immobilizing bacterial sensors.
Purpose of the Study:
- To develop a hydrogel microbead-based bacterial sensor for enhanced detection of nitro compounds.
- To improve the efficiency and signal amplification of bacterial sensors for remote explosive detection.
Main Methods:
- Genetically engineered Escherichia coli producing green fluorescent protein were loaded into poly(2-hydroxyethyl methacrylate) [poly(HEMA)]-based hydrogel beads.
- Surface charge of poly(HEMA) beads was modified using copolymerization with 2-(methacryloyloxy)ethyltrimethylammonium chloride (MAETC) to enhance bacterial loading.
- Bacterial sensors were exposed to 2,4,6-trinitrotoluene to assess fluorescence signal changes.
Main Results:
- Copolymerization with MAETC enhanced bacterial cell affinity ninefold due to electrostatic interactions.
- The bacterial sensor using poly(HEMA-co-MAETC) beads showed a fivefold increase in fluorescence intensity compared to poly(HEMA) beads after exposure to 2,4,6-trinitrotoluene.
- The amplified fluorescent signal facilitates easier remote detection of explosives.
Conclusions:
- Hydrogel microbeads functionalized with cationic monomers provide an effective platform for immobilizing bacterial sensors.
- The developed bacterial sensor demonstrates enhanced sensitivity and signal amplification for nitro compound detection.
- This technology holds promise for efficient remote detection of explosives.
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