Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phase II Reactions: Methylation Reactions01:17

Phase II Reactions: Methylation Reactions

704
Methylation is a phase II biotransformation process involving the attachment of a methyl group to a substrate. Enzymes known as methyltransferases orchestrate this reaction.
The mechanism of methylation unfolds in two stages. The first stage sees a methyltransferase enzyme facilitating the transfer of a methyl group from S-adenosylmethionine (SAM) to the substrate, forming S-adenosylhomocysteine (SAH). The second stage involves further metabolism of SAH into homocysteine, which can be recycled...
704
DNA Topoisomerases02:02

DNA Topoisomerases

35.3K
Topoisomerases are enzymes that relax overwound DNA molecules during various cell processes, including DNA replication and transcription. These enzymes regulate positive and negative DNA supercoiling without changing the nucleotide sequence. DNA overwinding in a clockwise direction results in positively supercoiled DNA, whereas underwinding in a counterclockwise direction produces negatively supercoiled DNA.
Types and Mechanism of action
Topoisomerases are divided into two main types. ...
35.3K
DNA Helicases00:55

DNA Helicases

24.0K
DNA unwinding helicase enzymes are a type of motor protein. Motor proteins can translocate along filaments or polymers using energy generated from ATP hydrolysis. Helicases are involved in all the important cellular processes where DNA unwinding is required, such as DNA replication, repair, recombination, and transcription. They are present in all living organisms, but vary in their structure, function, and mechanism of action. For example, in prokaryotes, DnaB helicase binds and translocates...
24.0K
Recombinant DNA01:09

Recombinant DNA

102.2K
Overview
102.2K
DNA Replication02:40

DNA Replication

59.1K
DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied.  After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
Replication in Prokaryotes
DNA replication...
59.1K
DNA-only Transposons02:57

DNA-only Transposons

17.4K
DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
17.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Corrigendum to: Vitamin D status and breast cancer in Saudi Arabian women: case-control study [Am J Clin Nutr 98 (2013) 105-110].

The American journal of clinical nutrition·2026
Same author

Per- and Polyfluoroalkyl Substances (PFAS) Exposure Profiles and Their Predictors in a Study of US Volunteer Firefighters.

American journal of industrial medicine·2026
Same author

Unveiling post-vaccination proteomic signatures in SARS-CoV-2 infection-naïve individuals associated with Omicron breakthrough infections.

PloS one·2026
Same author

Serum PFAS in Aircraft Rescue and Firefighting (ARFF) Firefighters From Six U.S. Airport Fire Departments.

American journal of industrial medicine·2026
Same author

Differences in MicroRNA Expression in Firefighters Responding to a Train Derailment and Fire in East Palestine, Ohio.

Epigenomes·2026
Same author

Secretory acid sphingomyelinase activity is elevated in persons with colorectal neoplasia.

medRxiv : the preprint server for health sciences·2026
Same journal

Analysis of strength degradation of coal and rock masses and stability of mined areas under long term immersion environment.

PloS one·2026
Same journal

Biogenic Silver-Selenium nanocomposite with anticancer activity and potent efficacy against vancomycin-resistant Staphylococcus aureus.

PloS one·2026
Same journal

Preparation and physicochemical characterization of a biodegradable chitosan/carboxymethyl cellulose hydrogel synthesized in NaOH/urea medium.

PloS one·2026
Same journal

Action-guilt, survivor-guilt, and depression in combat-related PTSD.

PloS one·2026
Same journal

Explainable machine learning for predicting activities of daily living at discharge in stroke patients: A retrospective study using SHAP interpretability.

PloS one·2026
Same journal

Deep learning based two-way feature depiction model for brain tumor detection.

PloS one·2026
See all related articles

Related Experiment Video

Updated: Jan 27, 2026

Methylated DNA Immunoprecipitation
21:24

Methylated DNA Immunoprecipitation

Published on: January 2, 2009

24.2K

DNA methylation among firefighters.

Jin Zhou1, Timothy G Jenkins2, Alesia M Jung1

  • 1Department of Epidemiology and Biostatistics, Mel and Enid Zuckerman College of Public Health, University of Arizona, Tucson, Arizona, United States of America.

Plos One
|March 27, 2019
PubMed
Summary
This summary is machine-generated.

Firefighter occupational exposures may alter DNA methylation, potentially increasing cancer risk. This study identified specific DNA methylation changes and pathways linked to firefighter status and service duration.

More Related Videos

DNA Methylation: Bisulphite Modification and Analysis
12:34

DNA Methylation: Bisulphite Modification and Analysis

Published on: October 21, 2011

106.4K
Targeted DNA Methylation Analysis by Next-generation Sequencing
08:38

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

38.0K

Related Experiment Videos

Last Updated: Jan 27, 2026

Methylated DNA Immunoprecipitation
21:24

Methylated DNA Immunoprecipitation

Published on: January 2, 2009

24.2K
DNA Methylation: Bisulphite Modification and Analysis
12:34

DNA Methylation: Bisulphite Modification and Analysis

Published on: October 21, 2011

106.4K
Targeted DNA Methylation Analysis by Next-generation Sequencing
08:38

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

38.0K

Area of Science:

  • Environmental Health
  • Epigenetics
  • Cancer Research

Background:

  • Firefighters face occupational carcinogen exposure.
  • Elevated cancer incidence is observed in the firefighting profession.

Purpose of the Study:

  • To investigate DNA methylation changes in firefighters due to occupational exposures.
  • To identify cancer-related pathways affected by these epigenetic alterations.

Main Methods:

  • Collected peripheral blood from 45 incumbent and 41 new recruit male firefighters.
  • Analyzed DNA methylation using an Illumina Methylation EPIC 850k chip.
  • Performed genome-wide differential methylation analysis, prediction modeling, and Ingenuity Pathway Analysis (IPA).

Main Results:

  • Identified four differentially methylated CpGs, including in YIPF6, MPST, and PCED1B genes.
  • Genome-wide methylation accurately predicted firefighter status and years of service.
  • IPA highlighted Sirtuin signaling, p53 signaling, and AMPK signaling pathways.

Conclusions:

  • DNA methylation patterns in firefighters may indicate cellular mechanisms contributing to cancer risk.
  • Epigenetic modifications are associated with occupational exposures in this cohort.
  • Findings suggest potential biomarkers and therapeutic targets for firefighter cancer prevention.