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Related Experiment Videos

Actin may be present on the lymphocyte surface.

M J Owen, J Auger, B H Barber

    Proceedings of the National Academy of Sciences of the United States of America
    |September 1, 1978
    PubMed
    Summary
    This summary is machine-generated.

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    Actin and myosin were investigated on lymphocyte surfaces. While initial findings suggested their presence on B and T cells, further analysis revealed cross-reactivity with immunoglobulin, indicating misleading immunofluorescence results.

    Area of Science:

    • Immunology
    • Cell Biology
    • Biochemistry

    Background:

    • Lymphocytes play crucial roles in the immune system.
    • Cell surface proteins are vital for lymphocyte function and recognition.

    Purpose of the Study:

    • To investigate the presence and localization of actin and myosin on the surface of lymphocytes.
    • To determine if these proteins are associated with immunoglobulin (Ig) on B lymphocytes.

    Main Methods:

    • Lactoperoxidase-catalyzed iodination was used to label surface proteins.
    • Indirect immunofluorescence with antisera against actin and myosin was employed.
    • Sodium dodecyl sulfate/polyacrylamide gel electrophoresis (SDS-PAGE) analyzed labeled proteins.
    • Binding assays with myosin and lentil lectin-Sepharose assessed protein characteristics.

    Related Experiment Videos

    Main Results:

    • SDS-PAGE revealed an intense radioactive band of 43,000 molecular weight in B lymphocytes, comigrating with actin.
    • T lymphocytes showed a less intense band compared to B lymphocytes.
    • Immunofluorescence staining of Ig-bearing cells with anti-actin and anti-myosin antisera was observed.
    • This staining was depleted when antisera were adsorbed with Ig-Sepharose, suggesting cross-reactivity.

    Conclusions:

    • Initial findings suggested actin on B and T lymphocyte surfaces.
    • Immunofluorescence results were deemed misleading due to antibody cross-reactivity with immunoglobulin.
    • The study highlights the importance of rigorous validation in surface protein analysis.