Challenge of Pasteurized Process Cheese Spreads with Clostridium botulinum Using In-Process and Post-Process
1Food Research Institute, Department of Food Microbiology and Toxicology, University of Wisconsin, Madison, Wisconsin 53706.
Journal of Food Protection
|March 28, 2019
Summary
This study evaluated the antibotulinal safety of pasteurized process cheese spreads. Cold inoculation methods revealed potential toxicity not detected by hot inoculation, highlighting critical safety differences.
Area of Science:
- Food Science
- Microbiology
- Food Safety
Background:
- Pasteurized process cheese spreads require robust safety evaluations against foodborne pathogens.
- Clostridium botulinum poses a significant risk, necessitating accurate challenge study methodologies.
Purpose of the Study:
- To assess the antibotulinal safety of pasteurized process cheese spreads.
- To compare the efficacy of two inoculation methods for challenging cheese spreads with Clostridium botulinum spores.
Main Methods:
- Pasteurized process cheese spreads were inoculated with Clostridium botulinum types A and B spores (approx. 1,000 spores/g).
- Two inoculation methods were compared: 'hot' (in-process) and 'cold' (post-process) addition of spore suspensions.
- Cold inoculation involved adding heat-shocked spores to jars of cheese spread followed by stirring.
Main Results:
- Some cheese spreads deemed safe by hot inoculation showed toxicity when subjected to cold inoculation.
- Experiments indicated spore localization and localized water dilution effects in cold-inoculated samples.
- Discrepancies in safety assessment were observed between the two inoculation methods.
Conclusions:
- The method of Clostridium botulinum spore inoculation significantly impacts the assessment of antibotulinal safety in pasteurized process cheese spreads.
- Cold inoculation methods may reveal safety vulnerabilities missed by hot inoculation techniques.
- Further research into spore localization and dilution effects is warranted for accurate food safety challenge studies.
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