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Published on: November 4, 2016
Inhibition and Inactivation of Staphylococcus aureus in a Sorbate/Modified Atmosphere Combination System
P H Elliott1, R I Tomlins1, R J H Gray1
1Department of Food Science and Human Nutrition, University of Delaware, Newark, Delaware 19711.
Abstract:
The combined effect of potassium sorbate, atmosphere composition and medium pH on viability of Staphylococcus aureus FDA S-6 was studied. Trypticase Soy Agar (TSA) containing 0.0, 0.5, 1.5 or 2.5% potassium sorbate was adjusted to pH 6.5, 6.0 or 5.5. S. aureus was exposed as a surface culture on the TSA and incubation was in a 100, 60 or 20% CO2 atmosphere; vacuum or air control. Modest inactivation of S. aureus was achieved in the absence of potassium sorbate at pH 5.5, when exposure was in a 100% CO2 atmosphere. In the presence of potassium sorbate, all atmospheres were inhibitory, with increased inactivation occurring at higher concentrations of sorbate and lower pH. Synergistic antimicrobial activity was optimal at pH 5.5 in the 1.5% sorbate/100% CO2 treatment.
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