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Updated: Jan 27, 2026

Assays for the Degradation of Misfolded Proteins in Cells
Published on: August 28, 2016
Urinary protein carbonyl levels and its correlation with protein misfolding in preeclampsia
Chandrakala Nagarajappa1, Sheela Shikaripur Rangappa2, Ravishankar Suryanarayana3
1a Department of Cell Biology and Molecular Genetics , Sri Devaraj Urs Academy of Higher Education and Research , Kolar , India.
Objective:
To evaluate the association of protein carbonylation with preeclampsia and its correlation with urinary protein misfolding.
Method:
Protein carbonyl and misfolded protein levels were measured in the midstream urine sample (58 preeclamptic and 44 normotensive pregnancy) by ELISA and Congo Red Dot assay respectively.
Results:
Significant difference was observed in the levels of protein carbonyls (P = 0.002) and misfolded proteins (P = 0.001). Correlation between protein carbonyl and misfolded proteins levels was significant but weak (r = 0.3; P = 0.018).
Conclusion:
Urinary protein carbonyl level is elevated in preeclampsia but plays a minor role in proteins misfolding.
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